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Effect of the CLSM pinhole

Pinholeseries 1.jpg Pinholeseries 2.jpg Pinholeseries 3.jpg Pinholeseries 4.jpg Pinholeseries 5.jpg Pinholeseries 6.jpg Pinholeseries 7.jpg Pinholeseries 8.jpg Pinholeseries 9.jpg


Cleaning an objective makes sense

  • First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.

dirty clean

  • Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm
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