Gallery

Effect of the CLSM pinhole

• The same region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).

• Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-580nm detection, 0.2µm Z-stepsize, Bitplane Imaris

Cleaning an objective makes sense

• First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.

• Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm