Gallery

Effect of the CLSM pinhole

overview images

• Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit.

• Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection

XY

• High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units.

• Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection

XYZ

• One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).

• Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris

Cleaning an objective makes sense

• First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.

• Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm