Grid LSM & WF, upr., BIOTEC
From BioDIP
(Difference between revisions)
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=05 |
| − | | system-name | + | |system-name=Visitron Vt-Infinity III grid scanning confocal |
| − | | category | + | |type=Microscope |
| − | | | + | |category=Real-Time Confocal |
| − | | | + | |facility=Imaging@Biotec |
| − | | | + | |building=Biotec |
| − | | facility_image | + | |room=227 |
| − | + | |facility_image=logo-imagingatbiotec.jpg | |
| − | + | |description=Our Vt-Infinity III is dedicated for high speed confocal imaging in XYZ. The laser lines allow GFP and RFP imaging, and the HBO allows common dye combinations. An EMCCD allows very fast stream acquisition of low signal. A dual-view system allows simultaneous detection of GFP and RFP in confocal mode. The use of dipping lenses makes this system especially suitable for study of embryo development. | |
| − | | description | + | |applications=- |
| − | | | + | |image=Bioz05.jpg |
| − | | | + | |stand=Upright |
| − | | illumination | + | |microscope=motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC |
| − | | detection | + | |obj1=Zeiss EC Plan-Neofluar 10x 0.3 |
| − | | reflectors | + | |obj2=Zeiss N-Achroplan 20x 0.5 W |
| − | | features | + | |obj3=Zeiss Achroplan 40x 0.8 W |
| − | | incubation | + | |obj4=Zeiss EC Plan-Neofluar 40x 1.3 Oil |
| + | |obj5=Zeiss Achroplan 63x 0.95 W | ||
| + | |obj6=Zeiss Plan-Apochromat 100x 1.4 Oil | ||
| + | |obj7=- | ||
| + | |obj8=- | ||
| + | |illumination=HBO lamp<br>Halogen lamp<br>491nm DPSS laser<br>561nm DPSS laser | ||
| + | |detection=widefield fluorescence with EMCCD, multi point scanner with EMCCD, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, variable size pinholes for confocal detection | ||
| + | |reflectors=DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>DsRed : EX 545/30 ; BS 570 ; EM 620/60<br>Scanhead Dicroics : GFP LP, Dual Band filter (491nm 561nm)<br>Scanhead Emission Filters : GFP LP, GFP 525/50, Dual Band (491nm 561nm)<br>Dual View Splitter : for simultaneous GFP/RPF detection : Double BP GFP/DsRed (Dic 565 ; EM 525/50 ; EM 630/75) | ||
| + | |features=widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing | ||
| + | |incubation=stage incubator (T) | ||
| + | |inv=0 | ||
| + | |objectives=10x/0.3<br>20x/0.5 W Ph2(Dipping)<br>40x/0.8 W (Dipping)<br>40x/3 Oil DIC<br>63x/1.3 Oil DIC<br>63x/0.95 W Ph3 (Dipping)<br>100x/1.4 Oil DIC | ||
}} | }} | ||
Revision as of 14:00, 4 September 2009
|
Directions
[[image:|150px|text-bottom|right]]
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | , Upright stand, motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination |
| ||
| detection | widefield fluorescence with EMCCD, multi point scanner with EMCCD, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, variable size pinholes for confocal detection | ||
| reflectors | DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50 GFP : EX 470/40 ; BS 495 ; EM 525/50 DsRed : EX 545/30 ; BS 570 ; EM 620/60 Scanhead Dicroics : GFP LP, Dual Band filter (491nm 561nm) Scanhead Emission Filters : GFP LP, GFP 525/50, Dual Band (491nm 561nm) Dual View Splitter : for simultaneous GFP/RPF detection : Double BP GFP/DsRed (Dic 565 ; EM 525/50 ; EM 630/75) | ||
| features | widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing | ||
| software | |||
| incubation | stage incubator (T) | ||
| links |
| ||
| inv.nr. | 0 |
