Grid LSM & WF, upr., BIOTEC
From BioDIP
(Difference between revisions)
| Line 7: | Line 7: | ||
|building=Biotec | |building=Biotec | ||
|room=227 | |room=227 | ||
| − | | | + | |Facility-logo=Imaging@Biotec.jpg |
| − | | | + | |Institute-logo=Logo Biotec.jpg |
|description=Our Vt-Infinity III is dedicated for high speed confocal imaging in XYZ. The laser lines allow GFP and RFP imaging, and the HBO allows common dye combinations. An EMCCD allows very fast stream acquisition of low signal. A dual-view system allows simultaneous detection of GFP and RFP in confocal mode. The use of dipping lenses makes this system especially suitable for study of embryo development. | |description=Our Vt-Infinity III is dedicated for high speed confocal imaging in XYZ. The laser lines allow GFP and RFP imaging, and the HBO allows common dye combinations. An EMCCD allows very fast stream acquisition of low signal. A dual-view system allows simultaneous detection of GFP and RFP in confocal mode. The use of dipping lenses makes this system especially suitable for study of embryo development. | ||
|applications=- | |applications=- | ||
|image=Bioz05.jpg | |image=Bioz05.jpg | ||
| + | |stand-name=Zeiss - Axio Imager | ||
|stand=Upright | |stand=Upright | ||
|microscope=motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC | |microscope=motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC | ||
| Line 26: | Line 27: | ||
|reflectors=Analysator<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>FITC : EX 480/20 ; BS 495 ; EM 535/40<br>TxRed : EX 570/20 ; BS 590 ; EM 640/40<br>DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50<br>empty<br>empty | |reflectors=Analysator<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>FITC : EX 480/20 ; BS 495 ; EM 535/40<br>TxRed : EX 570/20 ; BS 590 ; EM 640/40<br>DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50<br>empty<br>empty | ||
|features=widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing | |features=widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing | ||
| + | |software=- | ||
|incubation=Stage incubator (T) | |incubation=Stage incubator (T) | ||
|inv=0 | |inv=0 | ||
| + | |facility_image=Imaging@Biotec.jpg | ||
| + | |logo_image=Logo_Biotec.jpg | ||
|objectives=10x/0.3<br>20x/0.5 W Ph2(Dipping)<br>40x/0.8 W (Dipping)<br>40x/3 Oil DIC<br>63x/1.3 Oil DIC<br>63x/0.95 W Ph3 (Dipping)<br>100x/1.4 Oil DIC | |objectives=10x/0.3<br>20x/0.5 W Ph2(Dipping)<br>40x/0.8 W (Dipping)<br>40x/3 Oil DIC<br>63x/1.3 Oil DIC<br>63x/0.95 W Ph3 (Dipping)<br>100x/1.4 Oil DIC | ||
|illumination=HBO lamp<br>Halogen lamp<br>491nm DPSS laser<br>561nm DPSS laser | |illumination=HBO lamp<br>Halogen lamp<br>491nm DPSS laser<br>561nm DPSS laser | ||
}} | }} | ||
Revision as of 17:29, 26 November 2010
|
Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | Zeiss - Axio Imager, Upright stand, motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination | |||
| detection | widefield fluorescence with EMCCD, multi point scanner with EMCCD, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, variable size pinholes for confocal detection | ||
| reflectors | Analysator GFP : EX 470/40 ; BS 495 ; EM 525/50 FITC : EX 480/20 ; BS 495 ; EM 535/40 TxRed : EX 570/20 ; BS 590 ; EM 640/40 DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50 empty empty | ||
| features | widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing | ||
| software | - | ||
| incubation | Stage incubator (T) | ||
| links |
| ||
| inv.nr. | 0 |
