Grid LSM & WF, upr., BIOTEC
From BioDIP
(Difference between revisions)
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| objectives = 10x/0.3<br>20x/0.5 W Ph2(Dipping)<br>40x/0.8 W (Dipping)<br>40x/3 Oil DIC<br>63x/1.3 Oil DIC<br>63x/0.95 W Ph3 (Dipping)<br>100x/1.4 Oil DIC | | objectives = 10x/0.3<br>20x/0.5 W Ph2(Dipping)<br>40x/0.8 W (Dipping)<br>40x/3 Oil DIC<br>63x/1.3 Oil DIC<br>63x/0.95 W Ph3 (Dipping)<br>100x/1.4 Oil DIC | ||
| excitation = HBO lamp<br>Halogen lamp<br>491nm DPSS laser<br>561nm DPSS laser | | excitation = HBO lamp<br>Halogen lamp<br>491nm DPSS laser<br>561nm DPSS laser | ||
− | | detection = widefield fluorescence with EMCCD, multi point scanner with EMCCD, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, variable size pinholes for confocal detection, | + | | detection = widefield fluorescence with EMCCD, multi point scanner with EMCCD, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, variable size pinholes for confocal detection |
+ | | reflectors = DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>DsRed : EX 545/30 ; BS 570 ; EM 620/60<br>Scanhead Dicroics : GFP LP, Dual Band filter (491nm 561nm)<br>Scanhead Emission Filters : GFP LP, GFP 525/50, Dual Band (491nm 561nm)<br>Dual View Splitter : for simultaneous GFP/RPF detection : Double BP GFP/DsRed (Dic 565 ; EM 525/50 ; EM 630/75) | ||
| features = widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing | | features = widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing | ||
| incubation = Temperature control available on requirement (Bachhofer Chamber) | | incubation = Temperature control available on requirement (Bachhofer Chamber) | ||
}} | }} |
Revision as of 15:16, 16 June 2008
[[image:|250px]] |
Directions
[[image:|150px|text-bottom|right]]
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
microscope | , stand, Upright stand, motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC | ||
objectives |
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illumination |
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detection | widefield fluorescence with EMCCD, multi point scanner with EMCCD, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, variable size pinholes for confocal detection | ||
reflectors | DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50 GFP : EX 470/40 ; BS 495 ; EM 525/50 DsRed : EX 545/30 ; BS 570 ; EM 620/60 Scanhead Dicroics : GFP LP, Dual Band filter (491nm 561nm) Scanhead Emission Filters : GFP LP, GFP 525/50, Dual Band (491nm 561nm) Dual View Splitter : for simultaneous GFP/RPF detection : Double BP GFP/DsRed (Dic 565 ; EM 525/50 ; EM 630/75) | ||
features | widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing | ||
software | |||
incubation | Temperature control available on requirement (Bachhofer Chamber) | ||
links |
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inv.nr. |
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