Grid LSM & WF, upr., BIOTEC

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| objectives          = 10x/0.3<br>20x/0.5 W Ph2(Dipping)<br>40x/0.8 W (Dipping)<br>40x/3 Oil DIC<br>63x/1.3 Oil DIC<br>63x/0.95 W Ph3 (Dipping)<br>100x/1.4 Oil DIC
 
| objectives          = 10x/0.3<br>20x/0.5 W Ph2(Dipping)<br>40x/0.8 W (Dipping)<br>40x/3 Oil DIC<br>63x/1.3 Oil DIC<br>63x/0.95 W Ph3 (Dipping)<br>100x/1.4 Oil DIC
 
| excitation          =  HBO lamp<br>Halogen lamp<br>491nm DPSS laser<br>561nm DPSS laser
 
| excitation          =  HBO lamp<br>Halogen lamp<br>491nm DPSS laser<br>561nm DPSS laser
| detection            = widefield fluorescence with EMCCD, multi point scanner with EMCCD, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, variable size pinholes for confocal detection, dual-view for simultaneous GFP/RPF detection
+
| detection            = widefield fluorescence with EMCCD, multi point scanner with EMCCD, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, variable size pinholes for confocal detection
 +
| reflectors          = DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>DsRed : EX 545/30 ; BS 570 ; EM 620/60<br>Scanhead Dicroics : GFP LP, Dual Band filter (491nm 561nm)<br>Scanhead Emission Filters : GFP LP, GFP 525/50, Dual Band (491nm 561nm)<br>Dual View Splitter : for simultaneous GFP/RPF detection : Double BP GFP/DsRed (Dic 565 ; EM 525/50 ; EM 630/75)
 
| features            = widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing
 
| features            = widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing
 
| incubation          = Temperature control available on requirement (Bachhofer Chamber)
 
| incubation          = Temperature control available on requirement (Bachhofer Chamber)
 
}}
 
}}

Revision as of 15:16, 16 June 2008

Suitable for text.
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Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] Imaging@Biotec"Imaging@Biotec" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. ("Biotec" is not in the list of possible values (BIOTEC, CRTD, DZNE, MTZ, MPI-CBG) for this property., 227)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope , stand, Upright stand, motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC
objectives
illumination
detection widefield fluorescence with EMCCD, multi point scanner with EMCCD, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, variable size pinholes for confocal detection
reflectors DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50
GFP : EX 470/40 ; BS 495 ; EM 525/50
DsRed : EX 545/30 ; BS 570 ; EM 620/60
Scanhead Dicroics : GFP LP, Dual Band filter (491nm 561nm)
Scanhead Emission Filters : GFP LP, GFP 525/50, Dual Band (491nm 561nm)
Dual View Splitter : for simultaneous GFP/RPF detection : Double BP GFP/DsRed (Dic 565 ; EM 525/50 ; EM 630/75)
features widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging, summarizing
software
incubation Temperature control available on requirement (Bachhofer Chamber)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr.



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