Grid LSM & WF, upr., BIOTEC
From BioDIP
(Difference between revisions)
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|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
|Institute-logo=Logo Biotec.jpg | |Institute-logo=Logo Biotec.jpg | ||
| − | |description= | + | |description=The Vt-Infinity III is dedicated for high speed confocal imaging in XYZ. The laser lines allow GFP and RFP imaging, and the HBO allows common dye combinations. An EMCCD allows very fast stream acquisition of low signal. A dual-view system allows simultaneous detection of GFP and RFP in confocal mode. The use of dipping lenses makes this system especially suitable for study of embryo development. |
|applications=- | |applications=- | ||
|image=Bioz05.jpg | |image=Bioz05.jpg | ||
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|ill3=Laser DPSS 488 nm | |ill3=Laser DPSS 488 nm | ||
|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
| − | |detection=widefield fluorescence | + | |detection=dual ccd configuration: |
| + | Coolsnap ES for widefield fluorescence | ||
| + | Cascade II EMCCD for confocal sectioning (multi point illumination, variable pinhole size), dual channel emission beamsplitter | ||
|reflectors=Analysator<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>FITC : EX 480/20 ; BS 495 ; EM 535/40<br>TxRed : EX 570/20 ; BS 590 ; EM 640/40<br>DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50<br>empty<br>empty | |reflectors=Analysator<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>FITC : EX 480/20 ; BS 495 ; EM 535/40<br>TxRed : EX 570/20 ; BS 590 ; EM 640/40<br>DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50<br>empty<br>empty | ||
| − | |features=widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging | + | |features=widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging |
|software=- | |software=- | ||
|incubation=Stage incubator (T) | |incubation=Stage incubator (T) | ||
Revision as of 16:32, 4 May 2011
|
Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | Zeiss - Axio Imager, Upright stand, motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination | |||
| detection | dual ccd configuration:
Coolsnap ES for widefield fluorescence Cascade II EMCCD for confocal sectioning (multi point illumination, variable pinhole size), dual channel emission beamsplitter | ||
| reflectors | Analysator GFP : EX 470/40 ; BS 495 ; EM 525/50 FITC : EX 480/20 ; BS 495 ; EM 535/40 TxRed : EX 570/20 ; BS 590 ; EM 640/40 DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50 empty empty | ||
| features | widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging | ||
| software | - | ||
| incubation | Stage incubator (T) | ||
| links |
| ||
| inv.nr. | 0 |
