Grid LSM & WF, upr., BIOTEC

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Line 25: Line 25:
 
|ill4=Laser DPSS 561 nm
 
|ill4=Laser DPSS 561 nm
 
|detection=dual ccd configuration:  
 
|detection=dual ccd configuration:  
Coolsnap ES for widefield fluorescence
+
Coolsnap ES for widefield fluorescence AND
 
Cascade II EMCCD for confocal sectioning (multi point illumination, variable pinhole size), dual channel emission beamsplitter
 
Cascade II EMCCD for confocal sectioning (multi point illumination, variable pinhole size), dual channel emission beamsplitter
 
|reflectors=Analysator<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>FITC : EX 480/20 ; BS 495 ; EM 535/40<br>TxRed : EX 570/20 ; BS 590 ; EM 640/40<br>DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50<br>empty<br>empty
 
|reflectors=Analysator<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>FITC : EX 480/20 ; BS 495 ; EM 535/40<br>TxRed : EX 570/20 ; BS 590 ; EM 640/40<br>DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50<br>empty<br>empty

Revision as of 16:32, 4 May 2011

Suitable for -.
Bioz05.jpg


Directions

Imaging@Biotec.jpg Imaging@Biotec"Imaging@Biotec" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. ("Biotec" is not in the list of possible values (BIOTEC, CRTD, DZNE, MTZ, MPI-CBG) for this property., 227)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axio Imager, Upright stand, motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC
objectives
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss Achroplan 40x 0.8 W
Zeiss LD C-Apochromat 40x 1.1 W
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss Plan-Apochromat 100x 1.4 Oil
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
Laser DPSS 488 nm
Laser DPSS 561 nm
detection dual ccd configuration:

Coolsnap ES for widefield fluorescence AND Cascade II EMCCD for confocal sectioning (multi point illumination, variable pinhole size), dual channel emission beamsplitter

reflectors Analysator
GFP : EX 470/40 ; BS 495 ; EM 525/50
FITC : EX 480/20 ; BS 495 ; EM 535/40
TxRed : EX 570/20 ; BS 590 ; EM 640/40
DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50
empty
empty
features widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging
software -
incubation Stage incubator (T)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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