Grid LSM & WF, upr., BIOTEC

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|Facility-logo=Imaging@Biotec.jpg
 
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|description=The Vt-Infinity III is dedicated for high speed confocal imaging in XYZ. The laser lines allow GFP and RFP imaging, and the HBO allows common dye combinations. An EMCCD allows very fast stream acquisition of low signal. A dual-view system allows simultaneous detection of GFP and RFP in confocal mode. The use of dipping lenses makes this system especially suitable for study of embryo development.
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|description=The Vt-Infinity III is dedicated for high speed confocal imaging in XYZ. The laser lines allow GFP and RFP imaging, the HBO allows common dye combinations. An EMCCD allows very fast stream acquisition of low signal. A dual-view system allows simultaneous detection of GFP and RFP in confocal mode. The use of dipping lenses makes this system especially suitable for study of embryo development.
 
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|applications=-
 
|image=Bioz05.jpg
 
|image=Bioz05.jpg

Latest revision as of 11:50, 22 May 2014

Suitable for -.
Bioz05.jpg


[edit] Directions

Biotec logo.png
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 227)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Zeiss - Axio Imager, Upright stand, motorized XY stage, Z-Piezo stage, fluorescence, transmitted light with manual DIC
objectives
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss Achroplan 40x 0.8 W
Zeiss LD C-Apochromat 40x 1.1 W
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss Plan-Apochromat 100x 1.4 Oil
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
Laser DPSS 488 nm
Laser DPSS 561 nm
detection dual ccd configuration:

Coolsnap ES for widefield fluorescence AND
Cascade II EMCCD for confocal sectioning (multi point illumination, variable pinhole size), dual channel emission beamsplitter

reflectors Analysator
GFP : EX 470/40 ; BS 495 ; EM 525/50
FITC : EX 480/20 ; BS 495 ; EM 535/40
TxRed : EX 570/20 ; BS 590 ; EM 640/40
DAPI (A) : EX 350/50 ; BS 400 ; EM LP 460/50
features widefield fluoerescence imaging, confocal imaging, simultaneous GFP/RFP imaging, sequential imaging, Z stacks, time-series, advanced time-series, averaging
software MetaMorph
incubation Stage incubator (T)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 143913



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