|obj4=Olympus UPlanSApochromat 100x 1.4 Oil

|ill2=Fluorescence (HBO)

|detection=Spinning disc scan head Yokogawa CSU-X1 (5000rpm): <br>dichromatic mirrors:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br> [http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/561-13x15x0.5 Triple band T-405/488/561] <br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T488-13x15x0.5 Single band T-488] <br>physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>Andor Dual camera port with two cameras: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um<br>Andor Clara cooled interline CCD; dexel size of chip: 6.45um<br>note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 1.97x in front of iXon or a 0.95x in front of Clara<br>image pixel size for use with iXon (measured Sept. 8th 2012): with 100xoil objective: 0.081um/px (optovar 1x), 0.041um/px (opt. 2x); with 60xW obj.: 0.136um/px (opt.1x), 0.068um/px (opt 2x); 10x: 0.811um/px (opt.1x), 0.407um/px (opt.2x) <br> pixel size with Clara (measured Sept. 8th 2012): 100x oil objective: 0.068um/px (opt 1x), 0.034um/px (opt2x); 60xW obj.: 0.114um/px (opt.1x), 0.057um/px (opt. 2x); 10x obj: 0.68um/px (opt. 1x), 0.34um/px (opt. 2x) <br> <br>old values: <br>projecting lens in front of iXon: was 2.475 before installation of TuCAM on Sept 4th 2012; was 2.39x before removal of DualView on Dec 21st 2011; was 2.067x before addition of the dual filter wheel on Apr 28th 2011, was 1.9x before addition of dual camera switch on June 30th 2010 <br> projecting lens in front of Clara: was 1.285x before installation of TuCAM on Sept 4th 2012; was 1.28x before removal of DualView on Dec 21st 2011; was 1.2x before addition of the dual filter wheel on Apr 28th 2011<br><br>image pixel size for use with iXon (measured Jan 2012): with 100xoil objective: 0.0646um/px (optovar 1x), 0.0322um/px (opt. 2x); with 60xW obj.: 0.108um/px (opt.1x), 0.054um/px (opt 2x); 60x oil obj.: 0.1074um/px (opt. 1x), 0.0537um/px (opt. 2x); 10x: 0.648um/px (opt.1x), 0.324um/px (opt.2x) <br> pixel size with Clara (measured Jan 2012): 100x oil objective: 0.05um/px (opt 1x), 0.025um/px (opt2x); 60xW obj.: 0.084um/px (opt.1x), 0.042um/px (opt. 2x); 60xoil obj.: 0.0834um/px (opt.1x), 0.0417um/px (opt. 2x); 10x obj: 0.502um/px (opt. 1x), 0.251um/px (opt. 2x) <br> image pixel size used with iXon (measured Ju;y 15th 2011): with 100x: 0.067(was 0.077um before Apr 2011); with 60x objective: 0.111 (was 0.129 before Apr 2011) (was 0.14um); 60x obj. and 2.0x optovar: was 0.0645um (was 0.07um); 40x obj.: was 0.194um (was 0.21um); 40x obj. and 2.0x opt.: was 0.0967um (was 0.11um) <br> pixel size with Clara (measured July 15th 2011, all with optovar 1x): 100x objective: 0.05um/px, 60x obj.: 0.084um/px, 10x obj: 0.511um/px <br>

|reflectors=Sutter filter wheel A: <br>position 0: 440/521/607/700 Quadband,  1: razor edge 488LP,  2: blocked,  3: razor edge 568LP,  4: Edge Basic longpass 635 LP,  5: BrightLine 445/45,  6: open,  7: polarizer for DIC,  8: 525/30,  9: BrightLine HC 607/36<br>Sutter filter wheel B: <br> position 0: empty/open, position 1: BL 470/22; positions 2 - 9: empty/open <br>microscope filter turret:<br> position 1: empty,  2: DIC,  3: tripple DAPI + GFP + RFP cube,  4: GFP,  5: RFP,  6: DAPI cube (X 345/10; D 380LP; M 445/40)

|features=objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!

|software=iQ 2.9 + iQ 3.1 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br>

connections: COM1 - stage; COM2xx - lasers; COM3 - CSU; COM4 - Sutter FW; COM5 - Olympus IX 81; COM6 - FRAPPA; AOTF - ALC401/501/601; DD A06/16

|incubation=Stage incubator (T) + objective heater