H3 Andor STORM Spinning disc
From BioDIP
(Difference between revisions)
| Line 1: | Line 1: | ||
{{Equipment | {{Equipment | ||
| − | |system-number=MPI- H3 | + | |system-number=MPI-H3 |
|system-name=Andor STORM Spinning Disc | |system-name=Andor STORM Spinning Disc | ||
|type=Microscope | |type=Microscope | ||
| − | |category= | + | |category= |
| − | |facility= | + | |facility= |
|building=MPI-CBG | |building=MPI-CBG | ||
|room=340 | |room=340 | ||
| Line 11: | Line 11: | ||
|description=Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: TM-LED, Andor AMH, 405nm diode laser + 488 nm solid-state laser Coherent Sapphire + 561 nm solid-state laser + 640 nm solid-state laser. | |description=Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: TM-LED, Andor AMH, 405nm diode laser + 488 nm solid-state laser Coherent Sapphire + 561 nm solid-state laser + 640 nm solid-state laser. | ||
|applications=confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution | |applications=confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution | ||
| − | |||
|stand-name=Nikon TiE | |stand-name=Nikon TiE | ||
|stand=Inverted | |stand=Inverted | ||
Revision as of 18:03, 3 February 2016
| [[image:|250px]] |
Directions
Booking
Details
| microscope | Nikon TiE, Inverted stand, motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, manual xy stage | ||
| objectives | |||
| illumination | |||
| detection | [[detection::Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: dichromatic mirror: Quad band T-405/488/568/647 physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um ]] | ||
| reflectors | Rotr filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36 | ||
| features | FRAPPA coupled in via backport | ||
| software | [[software::iQ 2.9 + iQ 3.3 (both with Python) iQ3 user guide connections: COM1 - ????; COM2xx - lasers; COM3 - ???; COM4 - ???; COM5 - ???; COM6 - ???; AOTF - ALC401/501/601; DD A06/16]] | ||
| incubation | Stage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. |
[[Category:|Inverted]] [[Category:|]]
