H3 Andor STORM Spinning disc

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|description=Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: TM-LED, Andor AMH, 405nm diode laser + 488 nm solid-state laser Coherent Sapphire + 561 nm solid-state laser + 640 nm solid-state laser.
 
|description=Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: TM-LED, Andor AMH, 405nm diode laser + 488 nm solid-state laser Coherent Sapphire + 561 nm solid-state laser + 640 nm solid-state laser.
 
|applications=confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution
 
|applications=confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution
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|image=2012 09 10 SD2 FRAPPA.jpg
 
 
|stand-name=Nikon TiE
 
|stand-name=Nikon TiE
 
|stand=Inverted
 
|stand=Inverted
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|microscope=motorized with PFS (hardware autofocus), 1.5 x Optovar, Prior NanoScanZ, Prior ProScanIII xy scanning stage
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|microscope=motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, motorized XY stage
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|obj1=Olympus UPlanSApochromat 10x 0.4
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|obj1=Nikon Plan-Apochromat 10x 0.45 DIC
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|obj2=Olympus UPlanSApochromat 60x 1.2 W
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|obj2=Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC
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|obj3=Olympus UPlanSApochromat 60x 1.35 Oil
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|obj3=Nikon Plan-Apochromat 60x 1.2 W
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|obj4=Olympus UPlanSApochromat 100x 1.4 Oil
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|obj4=Nikon Apo 100x/1.49 Oil DIC 0.13-0.20
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|obj5=Olympus UApochromat 40x 1.15 W
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|ill1=Transmitted Light (LED)
 
|ill1=Transmitted Light (LED)
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|ill2=Fluorescence (HBO)
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|ill2=Fluorescence (Metal Halide)
 
|ill3=Laser Diode 405 nm
 
|ill3=Laser Diode 405 nm
 
|ill4=Laser DPSS 488 nm
 
|ill4=Laser DPSS 488 nm
 
|ill5=Laser DPSS 561 nm
 
|ill5=Laser DPSS 561 nm
 
|ill6=Laser DPSS 640 nm
 
|ill6=Laser DPSS 640 nm
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|detection=Spinning disc scan head Yokogawa CSU-X1 (5000rpm): <br>dichromatic mirrors:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br> [http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/561-13x15x0.5 Triple band T-405/488/561] <br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T488-13x15x0.5 Single band T-488] <br>physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>Andor Dual camera port with two cameras: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um<br>Andor Clara cooled interline CCD; dexel size of chip: 6.45um<br>note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 1.97x in front of iXon or a 0.95x in front of Clara<br>image pixel size for use with iXon (measured Sept. 8th 2012): with 100xoil objective: 0.081um/px (optovar 1x), 0.041um/px (opt. 2x); with 60xW obj.: 0.136um/px (opt.1x), 0.068um/px (opt 2x); 10x: 0.811um/px (opt.1x), 0.407um/px (opt.2x) <br> pixel size with Clara (measured Sept. 8th 2012): 100x oil objective: 0.068um/px (opt 1x), 0.034um/px (opt2x); 60xW obj.: 0.114um/px (opt.1x), 0.057um/px (opt. 2x); 10x obj: 0.68um/px (opt. 1x), 0.34um/px (opt. 2x) <br> <br>old values: <br>projecting lens in front of iXon: was 2.475 before installation of TuCAM on Sept 4th 2012; was 2.39x before removal of DualView on Dec 21st 2011; was 2.067x before addition of the dual filter wheel on Apr 28th 2011, was 1.9x before addition of dual camera switch on June 30th 2010 <br> projecting lens in front of Clara: was 1.285x before installation of TuCAM on Sept 4th 2012; was 1.28x before removal of DualView on Dec 21st 2011; was 1.2x before addition of the dual filter wheel on Apr 28th 2011<br><br>image pixel size for use with iXon (measured Jan 2012): with 100xoil objective: 0.0646um/px (optovar 1x), 0.0322um/px (opt. 2x); with 60xW obj.: 0.108um/px (opt.1x), 0.054um/px (opt 2x); 60x oil obj.: 0.1074um/px (opt. 1x), 0.0537um/px (opt. 2x); 10x: 0.648um/px (opt.1x), 0.324um/px (opt.2x) <br> pixel size with Clara (measured Jan 2012): 100x oil objective: 0.05um/px (opt 1x), 0.025um/px (opt2x); 60xW obj.: 0.084um/px (opt.1x), 0.042um/px (opt. 2x); 60xoil obj.: 0.0834um/px (opt.1x), 0.0417um/px (opt. 2x); 10x obj: 0.502um/px (opt. 1x), 0.251um/px (opt. 2x) <br> image pixel size used with iXon (measured Ju;y 15th 2011): with 100x: 0.067(was 0.077um before Apr 2011); with 60x objective: 0.111 (was 0.129 before Apr 2011) (was 0.14um); 60x obj. and 2.0x optovar: was 0.0645um (was 0.07um); 40x obj.: was 0.194um (was 0.21um); 40x obj. and 2.0x opt.: was 0.0967um (was 0.11um) <br> pixel size with Clara (measured July 15th 2011, all with optovar 1x): 100x objective: 0.05um/px, 60x obj.: 0.084um/px, 10x obj: 0.511um/px <br>
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|detection=Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: <br>dichromatic mirror:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br>physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>camera: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um; magnification of camera projection lens: 1.52x<br>
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|reflectors=Sutter filter wheel A: <br>position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP,  5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30,  9: BrightLine HC 607/36<br>Sutter filter wheel B: <br> position 0: empty/open, position 1: BL 470/22; positions 2 - 9: empty/open <br>microscope filter turret:<br> position 1: empty,  2: DIC,  3: tripple DAPI + GFP + RFP cube,  4: GFP,  5: RFP,  6: DAPI cube (X 345/10; D 380LP; M 445/40)
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image pixel sizes:<br>
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|features=objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!
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with 10x air obj.: 1.048um/px (1x optovar); 0.699 um/px (1.5x opt.); with 20x Oil/Glyc/Water obj.: 0.524um/px (1x optovar); 0.349 um/px (1.5x opt.); with 60x W obj.: 0.175um/px (1x opt.), 0.116um/px (1.5x opt.); with 100x oil obj.: 0.105um/px (1x opt.), 0.0698um/px (1.5x opt.)
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|software=iQ 2.9 + iQ 3.1 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br>
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|reflectors=Rotr filter wheel: <br>position 0: 520/15, 1: 628/40, 2: 450/50 (447/60 until 2018-04-09), 3: 607/36, 4: dual 512/630,  5: LP 664 RU, 6: 525/30, 7: 716/40, 8: LP 488 razor edge,  9: 617/73<br>
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connections: COM1 - stage; COM2xx - lasers; COM3 - CSU; COM4 - Sutter FW; COM5 - Olympus IX 81; COM6 - FRAPPA; AOTF - ALC401/501/601; DD A06/16
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|features=FRAPPA coupled in via backport
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|incubation=Stage incubator (T) + objective heater
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|software=iQ 2.9 + iQ 3.4.1 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br>
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|link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsbCKRwsAwzYA
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connections: COM1 - FRAPPA; COM2xx - lasers; COM3 - Yokogawa CSU-X; COM4 - RotR; AOTF - ALC401/501/601; DD A06/16: Device 1 - Nikon TiE; AMH;
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|incubation=Stage incubator (T+CO2)
 
|link2=-
 
|link2=-
−
|inv=0  10.1.145.20
 
 
}}
 
}}

Latest revision as of 15:12, 11 May 2020

Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution.
[[image:|250px]]


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 340)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Nikon TiE, Inverted stand, motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, motorized XY stage
objectives
Nikon Plan-Apochromat 10x 0.45 DIC
Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC
Nikon Plan-Apochromat 60x 1.2 W
Nikon Apo 100x/1.49 Oil DIC 0.13-0.20
illumination
Transmitted Light (LED)
Fluorescence (Metal Halide)
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection [[detection::Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual:
dichromatic mirror:
Quad band T-405/488/568/647
physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)
camera:
Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um; magnification of camera projection lens: 1.52x

image pixel sizes:
with 10x air obj.: 1.048um/px (1x optovar); 0.699 um/px (1.5x opt.); with 20x Oil/Glyc/Water obj.: 0.524um/px (1x optovar); 0.349 um/px (1.5x opt.); with 60x W obj.: 0.175um/px (1x opt.), 0.116um/px (1.5x opt.); with 100x oil obj.: 0.105um/px (1x opt.), 0.0698um/px (1.5x opt.)]]

reflectors Rotr filter wheel:
position 0: 520/15, 1: 628/40, 2: 450/50 (447/60 until 2018-04-09), 3: 607/36, 4: dual 512/630, 5: LP 664 RU, 6: 525/30, 7: 716/40, 8: LP 488 razor edge, 9: 617/73
features FRAPPA coupled in via backport
software [[software::iQ 2.9 + iQ 3.4.1 (both with Python) iQ3 user guide

connections: COM1 - FRAPPA; COM2xx - lasers; COM3 - Yokogawa CSU-X; COM4 - RotR; AOTF - ALC401/501/601; DD A06/16: Device 1 - Nikon TiE; AMH;]]

incubation Stage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr.


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