H3 Andor STORM Spinning disc
From BioDIP
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{{Equipment | {{Equipment | ||
| − | |system-number=MPI- H3 | + | |system-number=MPI-H3 |
|system-name=Andor STORM Spinning Disc | |system-name=Andor STORM Spinning Disc | ||
|type=Microscope | |type=Microscope | ||
| Line 11: | Line 11: | ||
|description=Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: TM-LED, Andor AMH, 405nm diode laser + 488 nm solid-state laser Coherent Sapphire + 561 nm solid-state laser + 640 nm solid-state laser. | |description=Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: TM-LED, Andor AMH, 405nm diode laser + 488 nm solid-state laser Coherent Sapphire + 561 nm solid-state laser + 640 nm solid-state laser. | ||
|applications=confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution | |applications=confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution | ||
| − | |||
|stand-name=Nikon TiE | |stand-name=Nikon TiE | ||
|stand=Inverted | |stand=Inverted | ||
| − | |microscope=motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, | + | |microscope=motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, motorized XY stage |
|obj1=Nikon Plan-Apochromat 10x 0.45 DIC | |obj1=Nikon Plan-Apochromat 10x 0.45 DIC | ||
|obj2=Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC | |obj2=Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC | ||
| Line 25: | Line 24: | ||
|ill5=Laser DPSS 561 nm | |ill5=Laser DPSS 561 nm | ||
|ill6=Laser DPSS 640 nm | |ill6=Laser DPSS 640 nm | ||
| − | |detection=Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: <br>dichromatic mirror:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br>physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>camera: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um<br> | + | |detection=Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: <br>dichromatic mirror:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br>physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>camera: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um; magnification of camera projection lens: 1.52x<br> |
| − | |reflectors=Rotr filter wheel: <br>position 0: | + | image pixel sizes:<br> |
| + | with 10x air obj.: 1.048um/px (1x optovar); 0.699 um/px (1.5x opt.); with 20x Oil/Glyc/Water obj.: 0.524um/px (1x optovar); 0.349 um/px (1.5x opt.); with 60x W obj.: 0.175um/px (1x opt.), 0.116um/px (1.5x opt.); with 100x oil obj.: 0.105um/px (1x opt.), 0.0698um/px (1.5x opt.) | ||
| + | |reflectors=Rotr filter wheel: <br>position 0: 520/15, 1: 628/40, 2: 450/50 (447/60 until 2018-04-09), 3: 607/36, 4: dual 512/630, 5: LP 664 RU, 6: 525/30, 7: 716/40, 8: LP 488 razor edge, 9: 617/73<br> | ||
|features=FRAPPA coupled in via backport | |features=FRAPPA coupled in via backport | ||
| − | |software=iQ 2.9 + iQ 3. | + | |software=iQ 2.9 + iQ 3.4.1 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br> |
| − | connections: COM1 - | + | connections: COM1 - FRAPPA; COM2xx - lasers; COM3 - Yokogawa CSU-X; COM4 - RotR; AOTF - ALC401/501/601; DD A06/16: Device 1 - Nikon TiE; AMH; |
|incubation=Stage incubator (T+CO2) | |incubation=Stage incubator (T+CO2) | ||
|link2=- | |link2=- | ||
}} | }} | ||
Latest revision as of 15:12, 11 May 2020
| [[image:|250px]] |
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Nikon TiE, Inverted stand, motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, motorized XY stage | ||
| objectives | |||
| illumination | |||
| detection | [[detection::Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: dichromatic mirror: Quad band T-405/488/568/647 physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um; magnification of camera projection lens: 1.52x image pixel sizes: | ||
| reflectors | Rotr filter wheel: position 0: 520/15, 1: 628/40, 2: 450/50 (447/60 until 2018-04-09), 3: 607/36, 4: dual 512/630, 5: LP 664 RU, 6: 525/30, 7: 716/40, 8: LP 488 razor edge, 9: 617/73 | ||
| features | FRAPPA coupled in via backport | ||
| software | [[software::iQ 2.9 + iQ 3.4.1 (both with Python) iQ3 user guide connections: COM1 - FRAPPA; COM2xx - lasers; COM3 - Yokogawa CSU-X; COM4 - RotR; AOTF - ALC401/501/601; DD A06/16: Device 1 - Nikon TiE; AMH;]] | ||
| incubation | Stage incubator (T+CO2) | ||
| links |
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| inv.nr. |
