H3 Andor STORM Spinning disc

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|features=FRAPPA coupled in via backport
 
|features=FRAPPA coupled in via backport
 
|software=iQ 2.9 + iQ 3.4.1 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br>
 
|software=iQ 2.9 + iQ 3.4.1 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br>
−
connections: COM1 - ????; COM2xx - lasers; COM3 - ???; COM4 - ???; COM5 - ???; COM6 - ???; AOTF - ALC401/501/601; DD A06/16
+
connections: COM1 - FRAPPA; COM2xx - lasers; COM3 - Yokogawa CSU-X; COM4 - RotR; AOTF - ALC401/501/601; DD A06/16: Device 1 - Nikon TiE; AMH;
 
|incubation=Stage incubator (T+CO2)
 
|incubation=Stage incubator (T+CO2)
 
|link2=-
 
|link2=-
 
}}
 
}}

Latest revision as of 15:12, 11 May 2020

Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution.
[[image:|250px]]


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 340)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Nikon TiE, Inverted stand, motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, motorized XY stage
objectives
Nikon Plan-Apochromat 10x 0.45 DIC
Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC
Nikon Plan-Apochromat 60x 1.2 W
Nikon Apo 100x/1.49 Oil DIC 0.13-0.20
illumination
Transmitted Light (LED)
Fluorescence (Metal Halide)
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection [[detection::Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual:
dichromatic mirror:
Quad band T-405/488/568/647
physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)
camera:
Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um; magnification of camera projection lens: 1.52x

image pixel sizes:
with 10x air obj.: 1.048um/px (1x optovar); 0.699 um/px (1.5x opt.); with 20x Oil/Glyc/Water obj.: 0.524um/px (1x optovar); 0.349 um/px (1.5x opt.); with 60x W obj.: 0.175um/px (1x opt.), 0.116um/px (1.5x opt.); with 100x oil obj.: 0.105um/px (1x opt.), 0.0698um/px (1.5x opt.)]]

reflectors Rotr filter wheel:
position 0: 520/15, 1: 628/40, 2: 450/50 (447/60 until 2018-04-09), 3: 607/36, 4: dual 512/630, 5: LP 664 RU, 6: 525/30, 7: 716/40, 8: LP 488 razor edge, 9: 617/73
features FRAPPA coupled in via backport
software [[software::iQ 2.9 + iQ 3.4.1 (both with Python) iQ3 user guide

connections: COM1 - FRAPPA; COM2xx - lasers; COM3 - Yokogawa CSU-X; COM4 - RotR; AOTF - ALC401/501/601; DD A06/16: Device 1 - Nikon TiE; AMH;]]

incubation Stage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr.


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