H3 Andor STORM Spinning disc

Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution.

Directions

MPI-CBG LMF (MPI-CBG, 340)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Nikon TiE, Inverted stand, motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, manual xy stage
objectives	Nikon Plan-Apochromat 10x 0.45 DIC Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC Nikon Plan-Apochromat 60x 1.2 W Nikon Apo 100x/1.49 Oil DIC 0.13-0.20
illumination	Transmitted Light (LED) Fluorescence (Metal Halide) Laser Diode 405 nm Laser DPSS 488 nm Laser DPSS 561 nm Laser DPSS 640 nm
detection	[[detection::Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: dichromatic mirror: Quad band T-405/488/568/647 physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um ]]
reflectors	Rotr filter wheel: position 0: 440/521/607/700 Quadband, 1: razor edge 488LP, 2: blocked, 3: razor edge 568LP, 4: Edge Basic longpass 635 LP, 5: BrightLine 445/45, 6: open, 7: polarizer for DIC, 8: 525/30, 9: BrightLine HC 607/36
features	FRAPPA coupled in via backport
software	[[software::iQ 2.9 + iQ 3.3 (both with Python) iQ3 user guide connections: COM1 - ????; COM2xx - lasers; COM3 - ???; COM4 - ???; COM5 - ???; COM6 - ???; AOTF - ALC401/501/601; DD A06/16]]
incubation	Stage incubator (T+CO2)
links	[ manual] [ laser power values] [- beam path] [ advanced system check]
inv.nr.

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