H3 Andor STORM Spinning disc
From BioDIP
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(updating software and filter info in RotR filter wheel) |
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|ill6=Laser DPSS 640 nm | |ill6=Laser DPSS 640 nm | ||
|detection=Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: <br>dichromatic mirror:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br>physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>camera: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um<br> | |detection=Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: <br>dichromatic mirror:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br>physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>camera: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um<br> | ||
− | |reflectors=Rotr filter wheel: <br>position 0: 520/15, 1: 628/40, 2: | + | |reflectors=Rotr filter wheel: <br>position 0: 520/15, 1: 628/40, 2: 447/60, 3: 607/36, 4: dual 512/630, 5: LP 664 RU, 6: 525/30, 7: 716/40, 8: LP 488 razor edge, 9: 617/73<br> |
|features=FRAPPA coupled in via backport | |features=FRAPPA coupled in via backport | ||
|software=iQ 2.9 + iQ 3.4.1 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br> | |software=iQ 2.9 + iQ 3.4.1 (both with Python) [https://www.biodip.de/wiki/File:IQ3_User_Guide.pdf iQ3 user guide]<br> |
Revision as of 19:11, 3 March 2017
[[image:|250px]] |
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Nikon TiE, Inverted stand, motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, motorized XY stage | ||
objectives | |||
illumination | |||
detection | [[detection::Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: dichromatic mirror: Quad band T-405/488/568/647 physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um ]] | ||
reflectors | Rotr filter wheel: position 0: 520/15, 1: 628/40, 2: 447/60, 3: 607/36, 4: dual 512/630, 5: LP 664 RU, 6: 525/30, 7: 716/40, 8: LP 488 razor edge, 9: 617/73 | ||
features | FRAPPA coupled in via backport | ||
software | [[software::iQ 2.9 + iQ 3.4.1 (both with Python) iQ3 user guide connections: COM1 - ????; COM2xx - lasers; COM3 - ???; COM4 - ???; COM5 - ???; COM6 - ???; AOTF - ALC401/501/601; DD A06/16]] | ||
incubation | Stage incubator (T+CO2) | ||
links |
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inv.nr. |