L1 - Long Term Timelapse I

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|stand=Inverted
 
|stand=Inverted
 
|microscope=motorized XY stage, motorized focus drive
 
|microscope=motorized XY stage, motorized focus drive
|obj1=Zeiss EC Plan-Neofluar 5x 0.16
+
|obj1=Plan Neofluar 1.25x/0.035
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
+
|obj2=Zeiss LD Achroplan 20x 0.4 Ph2
|obj3=Zeiss LD Achroplan 20x 0.4 Ph2
+
|obj3=Zeiss LD Achroplan 40x 0.6 Ph2
|obj4=Zeiss LD Achroplan 40x 0.6 Ph2
+
|obj4=Zeiss LD Achroplan 63x 0.75 Ph2
|obj5=Zeiss LD Achroplan 63x 0.75 Ph2
+
|obj6=Zeiss Plan-Apochromat 100x 1.4 Oil
+
|obj7=Zeiss Plan Neofluar 1.25x/0.035
+
 
|ill1=Transmitted Light (Halogen)
 
|ill1=Transmitted Light (Halogen)
 
|ill2=Fluorescence (Metal Halide, PRIOR, 200W)
 
|ill2=Fluorescence (Metal Halide, PRIOR, 200W)

Revision as of 16:28, 18 October 2011

Suitable for Long term time lapse imaging over hours or days.
MPI17.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, U35)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axiovert 200M, Inverted stand, motorized XY stage, motorized focus drive
objectives
Plan Neofluar 1.25x/0.035
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss LD Achroplan 40x 0.6 Ph2
Zeiss LD Achroplan 63x 0.75 Ph2
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, PRIOR, 200W)
detection Princeton Instruments monochrome CCD 1024x1024 pixels (6.7 um²)
reflectors DIC
DAPI
eGFP
TexRed/mCherry
Cy5
features -
software Molecular Devices MetaMorph v. 7.1
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 402165 10.1.64.227



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