L2 - Long Term Timelapse II

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{{Equipment
 
{{Equipment
|system-number=MPI-L2
+
|system-number=MPI- L2
 
|system-name=Long Term Timelapse II
 
|system-name=Long Term Timelapse II
 
|type=Microscope
 
|type=Microscope
Line 7: Line 7:
 
|building=MPI-CBG
 
|building=MPI-CBG
 
|room=U50
 
|room=U50
|facility_image=logo-lmf.jpg
+
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 +
|Institute-logo=Logo MPI-CBG.jpg
 
|description=Motorized inverted wide-field system.
 
|description=Motorized inverted wide-field system.
 
|applications=long-term imaging of living cells in glass-bottom-dishes or -plates
 
|applications=long-term imaging of living cells in glass-bottom-dishes or -plates
|image=MPI18.jpg
+
|image=L2 - Long Term Timelapse II.jpg
 +
|stand-name=Zeiss - Axiovert 200M
 
|stand=Inverted
 
|stand=Inverted
|microscope=Zeiss Axiovert 200M, motorized XY stage, motorized focus drive
+
|microscope=motorized XY stage, motorized focus drive
|obj1=Zeiss EC Plan-Neofluar 5x 0.16 Ph1
+
|obj1=Zeiss Plan-Neofluar 5x 0.15 Ph1
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
+
|obj2=Zeiss Plan-Neofluar 10x 0.3 Ph1
|obj3=Zeiss LD Achroplan 20x 0.4 Ph2
+
|obj3=Zeiss LD-Achroplan 20x 0.4 Ph2
|obj4=Zeiss Plan-Apochromat 20x 0.8
+
|obj4=Zeiss Plan-Neofluar 40x 0.75 Ph2
|obj5=Zeiss LD Achroplan 63x 0.75 Ph2
+
|obj5=Zeiss LD-Achroplan 63x 0.75 Ph2
|obj6=Zeiss EC Plan-Neofluar 63x 1.25 Oil
+
|obj6=Zeiss Plan-Apochromat 100x 1.4 Oil DIC
 +
|obj7=Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water
 +
|obj8=Zeiss C-Apochromat 40x 1.2 W
 +
|obj9=Zeiss Plan-Neofluar 100x 1.3 Oil Ph3
 
|ill1=Transmitted Light (Halogen)
 
|ill1=Transmitted Light (Halogen)
 
|ill2=Fluorescence (Metal Halide)
 
|ill2=Fluorescence (Metal Halide)
 
|detection=Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
 
|detection=Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
|reflectors=DAPI<br>HC-Quad-Set Dapi AHF F68-888<br>GFP/DsRed<br>Dapi/Fitc/DsRed<br>ET GFP 49002
+
|reflectors=DAPI<br>HC-Quad-Set (AHF F68-888)<br>GFP/DsRed<br>Dapi/Fitc/DsRed<br>ET GFP 49002
 
|features=-
 
|features=-
 +
|software=Molecular Devices MetaMorph v. 7.1
 
|incubation=Stage incubator (T+CO2)
 
|incubation=Stage incubator (T+CO2)
 
|link1=-
 
|link1=-
 
|link2=-
 
|link2=-
|inv=404984
+
|inv=404984 10.1.145.18
 +
|facility_image=logo-lmf.jpg
 
|objectives=63x/1.25 Oil Plan-Neofluar<br>63x/0.75 LD Achroplan Ph2 corr<br>20x/0.8 Plan-Apochromat<br>20x/0.4 LD Achroplan Ph2 corr<br>10x/0.3 Plan-Neofluar Ph1<br>5x/0.15 Plan-Neofluar Ph1
 
|objectives=63x/1.25 Oil Plan-Neofluar<br>63x/0.75 LD Achroplan Ph2 corr<br>20x/0.8 Plan-Apochromat<br>20x/0.4 LD Achroplan Ph2 corr<br>10x/0.3 Plan-Neofluar Ph1<br>5x/0.15 Plan-Neofluar Ph1
 
|illumination=Halogen<br>X-Cite (metal halide)
 
|illumination=Halogen<br>X-Cite (metal halide)
 
}}
 
}}

Latest revision as of 17:37, 3 February 2016

Suitable for long-term imaging of living cells in glass-bottom-dishes or -plates.
L2 - Long Term Timelapse II.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, U50)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Zeiss - Axiovert 200M, Inverted stand, motorized XY stage, motorized focus drive
objectives
Zeiss Plan-Neofluar 5x 0.15 Ph1
Zeiss Plan-Neofluar 10x 0.3 Ph1
Zeiss LD-Achroplan 20x 0.4 Ph2
Zeiss Plan-Neofluar 40x 0.75 Ph2
Zeiss LD-Achroplan 63x 0.75 Ph2
Zeiss Plan-Apochromat 100x 1.4 Oil DIC
Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water
Zeiss C-Apochromat 40x 1.2 W
Zeiss Plan-Neofluar 100x 1.3 Oil Ph3
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide)
detection Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
reflectors DAPI
HC-Quad-Set (AHF F68-888)
GFP/DsRed
Dapi/Fitc/DsRed
ET GFP 49002
features -
software Molecular Devices MetaMorph v. 7.1
incubation Stage incubator (T+CO2)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 404984 10.1.145.18



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