L2 - Long Term Timelapse II

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{{Equipment
 
{{Equipment
| system-number       = MPI18
+
|system-number=MPI18
| system-name         = Long Term Timelapse II
+
|system-name=Long Term Timelapse II
| category             = Wide-Field
+
|type=Microscope
| stand                = inverted
+
|category=Wide-Field
| image                = MPI18.jpg
+
|facility=MPI-CBG LMF
| facility             = MPI-CBG LMF
+
|building=MPI-CBG
| facility_image      = logo-lmf.jpg
+
|room=U50
| building             = MPI-CBG
+
|facility_image=logo-lmf.jpg
| room                 = U50
+
|description=Motorized inverted wide-field system.
| description         = Motorized inverted wide-field system.
+
|applications=long-term imaging of living cells in glass-bottom-dishes or -plates
| applications         = long-term imaging of living cells in glass-bottom-dishes or -plates
+
|image=MPI18.jpg
| microscope           = Zeiss Axiovert 200M, motorized XY stage, motorized focus drive
+
|stand=Inverted
| objectives           = 63x/1.25 Oil Plan-Neofluar<br>63x/0.75 LD Achroplan Ph2 corr<br>20x/0.8 Plan-Apochromat<br>20x/0.4 LD Achroplan Ph2 corr<br>10x/0.3 Plan-Neofluar Ph1<br>5x/0.15 Plan-Neofluar Ph1
+
|microscope=Zeiss Axiovert 200M, motorized XY stage, motorized focus drive
| illumination         = Halogen<br>X-Cite (metal halide)
+
|objectives=63x/1.25 Oil Plan-Neofluar<br>63x/0.75 LD Achroplan Ph2 corr<br>20x/0.8 Plan-Apochromat<br>20x/0.4 LD Achroplan Ph2 corr<br>10x/0.3 Plan-Neofluar Ph1<br>5x/0.15 Plan-Neofluar Ph1
| detection           = Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
+
|illumination=Halogen<br>X-Cite (metal halide)
| reflectors           = DAPI<br>HC-Quad-Set Dapi AHF F68-888<br>GFP/DsRed<br>Dapi/Fitc/DsRed<br>ET GFP 49002
+
|detection=Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
| features             = -
+
|reflectors=DAPI<br>HC-Quad-Set Dapi AHF F68-888<br>GFP/DsRed<br>Dapi/Fitc/DsRed<br>ET GFP 49002
| incubation           = cage incubator with heating and CO2 control capabilities
+
|features=-
| link1               = -
+
|incubation=cage incubator (T+CO2)
| link2               = -
+
|link1=-
| inv                 = 404984
+
|link2=-
 +
|inv=404984
 +
|obj1=Zeiss EC Plan-Neofluar 5x 0.16 Ph1
 +
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
 +
|obj3=Zeiss LD Achroplan 20x 0.4 Ph2
 +
|obj4=Zeiss Plan-Apochromat 20x 0.8
 +
|obj5=Zeiss LD Achroplan 63x 0.75 Ph2
 +
|obj6=Zeiss EC Plan-Neofluar 63x 1.25 Oil
 +
|obj7=-
 +
|obj8=-
 
}}
 
}}

Revision as of 16:38, 3 September 2009

Suitable for long-term imaging of living cells in glass-bottom-dishes or -plates.
MPI18.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, U50)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope , Inverted stand, Zeiss Axiovert 200M, motorized XY stage, motorized focus drive
objectives
Zeiss EC Plan-Neofluar 5x 0.16 Ph1
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss Plan-Apochromat 20x 0.8
Zeiss LD Achroplan 63x 0.75 Ph2
Zeiss EC Plan-Neofluar 63x 1.25 Oil
-
-
illumination
detection Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
reflectors DAPI
HC-Quad-Set Dapi AHF F68-888
GFP/DsRed
Dapi/Fitc/DsRed
ET GFP 49002
features -
software
incubation cage incubator (T+CO2)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 404984



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