L2 - Long Term Timelapse II

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|stand=Inverted
 
|stand=Inverted
 
|microscope=Zeiss Axiovert 200M, motorized XY stage, motorized focus drive
 
|microscope=Zeiss Axiovert 200M, motorized XY stage, motorized focus drive
|objectives=63x/1.25 Oil Plan-Neofluar<br>63x/0.75 LD Achroplan Ph2 corr<br>20x/0.8 Plan-Apochromat<br>20x/0.4 LD Achroplan Ph2 corr<br>10x/0.3 Plan-Neofluar Ph1<br>5x/0.15 Plan-Neofluar Ph1
 
|illumination=Halogen<br>X-Cite (metal halide)
 
|detection=Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
 
|reflectors=DAPI<br>HC-Quad-Set Dapi AHF F68-888<br>GFP/DsRed<br>Dapi/Fitc/DsRed<br>ET GFP 49002
 
|features=-
 
|incubation=cage incubator (T+CO2)
 
|link1=-
 
|link2=-
 
|inv=404984
 
 
|obj1=Zeiss EC Plan-Neofluar 5x 0.16 Ph1
 
|obj1=Zeiss EC Plan-Neofluar 5x 0.16 Ph1
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
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|obj7=-
 
|obj7=-
 
|obj8=-
 
|obj8=-
 +
|ill1=Transmitted Light (Halogen)
 +
|ill2=Fluorescence (Metal Halide)
 +
|ill3=-
 +
|ill4=-
 +
|ill5=-
 +
|ill6=-
 +
|ill7=-
 +
|ill8=-
 +
|detection=Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
 +
|reflectors=DAPI<br>HC-Quad-Set Dapi AHF F68-888<br>GFP/DsRed<br>Dapi/Fitc/DsRed<br>ET GFP 49002
 +
|features=-
 +
|incubation=cage incubator (T+CO2)
 +
|link1=-
 +
|link2=-
 +
|inv=404984
 +
|objectives=63x/1.25 Oil Plan-Neofluar<br>63x/0.75 LD Achroplan Ph2 corr<br>20x/0.8 Plan-Apochromat<br>20x/0.4 LD Achroplan Ph2 corr<br>10x/0.3 Plan-Neofluar Ph1<br>5x/0.15 Plan-Neofluar Ph1
 +
|illumination=Halogen<br>X-Cite (metal halide)
 
}}
 
}}

Revision as of 15:57, 7 September 2009

Suitable for long-term imaging of living cells in glass-bottom-dishes or -plates.
MPI18.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, U50)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope , Inverted stand, Zeiss Axiovert 200M, motorized XY stage, motorized focus drive
objectives
Zeiss EC Plan-Neofluar 5x 0.16 Ph1
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss Plan-Apochromat 20x 0.8
Zeiss LD Achroplan 63x 0.75 Ph2
Zeiss EC Plan-Neofluar 63x 1.25 Oil
-
-
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide)
-
-
-
-
-
-
detection Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
reflectors DAPI
HC-Quad-Set Dapi AHF F68-888
GFP/DsRed
Dapi/Fitc/DsRed
ET GFP 49002
features -
software
incubation cage incubator (T+CO2)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 404984



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