L2 - Long Term Timelapse II

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Line 11: Line 11:
 
|applications=long-term imaging of living cells in glass-bottom-dishes or -plates
 
|applications=long-term imaging of living cells in glass-bottom-dishes or -plates
 
|image=MPI18.jpg
 
|image=MPI18.jpg
 +
|stand-name=Zeiss - Axiovert 200M
 
|stand=Inverted
 
|stand=Inverted
|microscope=Zeiss Axiovert 200M, motorized XY stage, motorized focus drive
+
|microscope=motorized XY stage, motorized focus drive
 
|obj1=Zeiss EC Plan-Neofluar 5x 0.16 Ph1
 
|obj1=Zeiss EC Plan-Neofluar 5x 0.16 Ph1
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1

Revision as of 17:53, 30 March 2010

Suitable for long-term imaging of living cells in glass-bottom-dishes or -plates.
MPI18.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, U50)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axiovert 200M, Inverted stand, motorized XY stage, motorized focus drive
objectives
Zeiss EC Plan-Neofluar 5x 0.16 Ph1
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss Plan-Apochromat 20x 0.8
Zeiss LD Achroplan 63x 0.75 Ph2
Zeiss EC Plan-Neofluar 63x 1.25 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide)
detection Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
reflectors DAPI
HC-Quad-Set (AHF F68-888)
GFP/DsRed
Dapi/Fitc/DsRed
ET GFP 49002
features -
software Molecular Devices MetaMorph v. 7.1
incubation Stage incubator (T+CO2)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 404984



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