L2 - Long Term Timelapse II

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m (moved Long Term Timelapse II to L2 - Long Term Timelapse II: to match LMF booking database)
Line 7: Line 7:
 
|building=MPI-CBG
 
|building=MPI-CBG
 
|room=U50
 
|room=U50
|facility_image=logo-lmf.jpg
 
 
|description=Motorized inverted wide-field system.
 
|description=Motorized inverted wide-field system.
 
|applications=long-term imaging of living cells in glass-bottom-dishes or -plates
 
|applications=long-term imaging of living cells in glass-bottom-dishes or -plates
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|stand=Inverted
 
|stand=Inverted
 
|microscope=motorized XY stage, motorized focus drive
 
|microscope=motorized XY stage, motorized focus drive
|obj1=Zeiss EC Plan-Neofluar 5x 0.16 Ph1
+
|obj1=Zeiss EC Plan-Neofluar 5x 0.15 Ph1
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
 
|obj3=Zeiss LD Achroplan 20x 0.4 Ph2
 
|obj3=Zeiss LD Achroplan 20x 0.4 Ph2
|obj4=Zeiss Plan-Apochromat 20x 0.8
+
|obj4=Zeiss EC Plan-Neofluar 40x 0.75
 
|obj5=Zeiss LD Achroplan 63x 0.75 Ph2
 
|obj5=Zeiss LD Achroplan 63x 0.75 Ph2
|obj6=Zeiss EC Plan-Neofluar 63x 1.25 Oil
+
|obj6=Zeiss Plan-Apochromat 100x 1.4 Oil
 
|ill1=Transmitted Light (Halogen)
 
|ill1=Transmitted Light (Halogen)
 
|ill2=Fluorescence (Metal Halide)
 
|ill2=Fluorescence (Metal Halide)
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|link2=-
 
|link2=-
 
|inv=404984
 
|inv=404984
 +
|facility_image=logo-lmf.jpg
 
|objectives=63x/1.25 Oil Plan-Neofluar<br>63x/0.75 LD Achroplan Ph2 corr<br>20x/0.8 Plan-Apochromat<br>20x/0.4 LD Achroplan Ph2 corr<br>10x/0.3 Plan-Neofluar Ph1<br>5x/0.15 Plan-Neofluar Ph1
 
|objectives=63x/1.25 Oil Plan-Neofluar<br>63x/0.75 LD Achroplan Ph2 corr<br>20x/0.8 Plan-Apochromat<br>20x/0.4 LD Achroplan Ph2 corr<br>10x/0.3 Plan-Neofluar Ph1<br>5x/0.15 Plan-Neofluar Ph1
 
|illumination=Halogen<br>X-Cite (metal halide)
 
|illumination=Halogen<br>X-Cite (metal halide)
 
}}
 
}}

Revision as of 15:25, 11 August 2011

Suitable for long-term imaging of living cells in glass-bottom-dishes or -plates.
MPI18.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, U50)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axiovert 200M, Inverted stand, motorized XY stage, motorized focus drive
objectives
Zeiss EC Plan-Neofluar 5x 0.15 Ph1
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss EC Plan-Neofluar 40x 0.75
Zeiss LD Achroplan 63x 0.75 Ph2
Zeiss Plan-Apochromat 100x 1.4 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide)
detection Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
reflectors DAPI
HC-Quad-Set (AHF F68-888)
GFP/DsRed
Dapi/Fitc/DsRed
ET GFP 49002
features -
software Molecular Devices MetaMorph v. 7.1
incubation Stage incubator (T+CO2)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 404984



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