L2 - Long Term Timelapse II

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Line 13: Line 13:
 
|stand=Inverted
 
|stand=Inverted
 
|microscope=motorized XY stage, motorized focus drive
 
|microscope=motorized XY stage, motorized focus drive
|obj1=Zeiss EC Plan-Neofluar 5x 0.15 Ph1
+
|obj1=Zeiss Plan-Neofluar 5x 0.15 Ph1
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
+
|obj2=Zeiss Plan-Neofluar 10x 0.3 Ph1
 
|obj3=Zeiss LD Achroplan 20x 0.4 Ph2
 
|obj3=Zeiss LD Achroplan 20x 0.4 Ph2
 
|obj4=Zeiss Plan-Neofluar 40x 0.75
 
|obj4=Zeiss Plan-Neofluar 40x 0.75

Revision as of 11:39, 30 August 2011

Suitable for long-term imaging of living cells in glass-bottom-dishes or -plates.
MPI18.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, U50)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axiovert 200M, Inverted stand, motorized XY stage, motorized focus drive
objectives
Zeiss Plan-Neofluar 5x 0.15 Ph1
Zeiss Plan-Neofluar 10x 0.3 Ph1
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss Plan-Neofluar 40x 0.75
Zeiss LD Achroplan 63x 0.75 Ph2
Zeiss Plan-Apochromat 100x 1.4 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide)
detection Roper Scientific CoolSNAP ES, monochromatic Sony ICX285AL chip, 1392 x 1040 pixels, 6.45 μm pixel size, 20 MHz digitization, 8 e- rms readout noise @ 20 MHz, thermoelectric cooling (5°C below ambient temperature)
reflectors DAPI
HC-Quad-Set (AHF F68-888)
GFP/DsRed
Dapi/Fitc/DsRed
ET GFP 49002
features -
software Molecular Devices MetaMorph v. 7.1
incubation Stage incubator (T+CO2)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 404984



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