L5 - Olympus ScanR+CellR

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{{Equipment
 
{{Equipment
 
|system-number=MPI-L5
 
|system-number=MPI-L5
|system-name=Olympus ScanR + CellR
+
|system-name=Olympus ScanR+CellR
|type=Microscope
+
|type=Not listed
 
|category=Wide-Field
 
|category=Wide-Field
 
|facility=MPI-CBG LMF
 
|facility=MPI-CBG LMF
 
|building=MPI-CBG
 
|building=MPI-CBG
 
|room=U50
 
|room=U50
|facility_image=logo-lmf.jpg
+
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 +
|Institute-logo=Logo MPI-CBG.jpg
 
|description=Motorized inverted wide-field system with temperature and CO2 cage incubator.
 
|description=Motorized inverted wide-field system with temperature and CO2 cage incubator.
 
|applications=long-term observation of living cells, Fast multi-fluorescence 3-D time-lapse, Fast and precise image acquisition and analysis, robust automated object detection and cell separation, visual correlation of cell images and analysis results.
 
|applications=long-term observation of living cells, Fast multi-fluorescence 3-D time-lapse, Fast and precise image acquisition and analysis, robust automated object detection and cell separation, visual correlation of cell images and analysis results.
|image=MPI24 .jpg
+
|image=L5 - Olympus ScanR CellR.jpg
 
|stand-name=Olympus - IX81
 
|stand-name=Olympus - IX81
 
|stand=Inverted
 
|stand=Inverted
 
|microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light
 
|microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light
|obj1=Olympus UPlanSApo 4x 0.16
+
|obj1=Olympus UPlan-SApochromat 40x 0.9
|obj2=Olympus UPlanSApo 40X 0.9
+
|obj2=Olympus UPlan-SApochromat 20x 0.75
|obj3=Olympus UPlanFluor 20X 0.5
+
|obj3=Olympus UPlan-SApochromat 10x 0.4
|obj4=Olympus UPlanSApo 40X 0.9
+
|obj4=Olympus UPlan-FluarN 20x 0.5 Ph1
|obj5=Olympus UPlanSApo 60x 1.35 Oil
+
|obj5=Olympus LCPlan-Fluar 20x 0.4 Ph1
|obj6=Olympus UPlanFluor 60x 1.25 Oil Ph3 Iris
+
|obj6=Olympus UPlan-FluarN 40x 0.75 Ph2
 +
|obj7=Olympus UPlan-SApochromat 4x 0.16
 +
|obj8=Olympus Uplan-Apochromat 40x 1 Oil
 +
|obj9=Olympus UPlan-SApochromat 60x 1.35 Oil
 +
|obj10=Olympus UPlan-Fluar 20x 0.5
 
|ill1=Transmitted Light (Halogen)
 
|ill1=Transmitted Light (Halogen)
 
|ill2=Fluorescence (Xenon)
 
|ill2=Fluorescence (Xenon)
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|link1=-
 
|link1=-
 
|link2=-
 
|link2=-
|inv=405679
+
|inv=405679 10.1.145.24
 +
|facility_image=logo-lmf.jpg
 
|objectives=#  UPlanSApo 4X N.A 0.16
 
|objectives=#  UPlanSApo 4X N.A 0.16
 
# UPlanSApo 10X N.A 0.4
 
# UPlanSApo 10X N.A 0.4

Latest revision as of 16:30, 3 February 2016

Suitable for long-term observation of living cells, Fast multi-fluorescence 3-D time-lapse, Fast and precise image acquisition and analysis, robust automated object detection and cell separation, visual correlation of cell images and analysis results..
L5 - Olympus ScanR CellR.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, U50)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Olympus - IX81, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light
objectives
Olympus UPlan-SApochromat 40x 0.9
Olympus UPlan-SApochromat 20x 0.75
Olympus UPlan-SApochromat 10x 0.4
Olympus UPlan-FluarN 20x 0.5 Ph1
Olympus LCPlan-Fluar 20x 0.4 Ph1
Olympus UPlan-FluarN 40x 0.75 Ph2
Olympus UPlan-SApochromat 4x 0.16
Olympus Uplan-Apochromat 40x 1 Oil
Olympus UPlan-SApochromat 60x 1.35 Oil
Olympus UPlan-Fluar 20x 0.5
illumination
Transmitted Light (Halogen)
Fluorescence (Xenon)
detection Hamamatsu Camera ORCA ER, progressive scan interline CCD, 1344 x 1024 pixels, 6.45 um pixel size, 14.75 MHz digitization, 8 e- rms readout noise, 8 fps @ full frame, 18.000 e- full well capacity, 2250:1 dynamic range, peltier cooling (-20 degrees C @ 20 degrees C ambient), 12 bit ADC, 0.1 electron/pixel/sec dark current, up to 10x analog gain, offset functions
reflectors MT20 Excitation Unit

- EXCITATION FILTERS
387 / 403 / 485 / 492 / 560 / 572/ 650

- DICHROICS AND EMISSION FILTERS

* POSITION 1:
D 405-435/450-485/510-550/590-675
M 450-475/515-545/600-665

* POSITION 2:
D QBP 410-465, 504-538, 581-632, 669-743
M QBP 415-461, 507-533, 586-625, 673-722

* POSITION 3: EMPTY

* POSITION 4: EMPTY

* POSITION 5:
D 485-570/605-690
M 500-560/615-675

* POSITION 6
D 440-490/510-575/610-675
M 450-480/525-560/615-660

features Dual System. It can work as Scan R or as CELL R
SCAN R
Modular microscope-based imaging platform designed for fully automated image acquisition and data analysis of biological samples. scan^R can handle many different formats e.g. multi-well plates, slides or custom-built arrays. The unmatched flexibility and open design make it equally adept at routine and advanced applications. With its powerful analysis module for biological functional assays, it is the perfect tool for assay development and high-content screening. This provides complex image analysis and advanced data evaluation.
CELL R
consists of an all-in-one illumination system MT20, highly sensitive digital cameras and a hyper-precision hardware control board."Dual System. It can work as Scan R or as CELL R <br />SCAN R <br /> Modular microscope-based imaging platform designed for fully automated image acquisition and data analysis of biological samples. scan^R can handle many different formats e.g. multi-well plates, slides or custom-built arrays. The unmatched flexibility and open design make it equally adept at routine and advanced applications. With its powerful analysis module for biological functional assays, it is the perfect tool for assay development and high-content screening. This provides complex image analysis and advanced data evaluation. <br /> CELL R<br /> consists of an all-in-one illumination system MT20, highly sensitive digital cameras and a hyper-precision hardware control board." cannot be used as a page name in this wiki.
software -
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 405679 10.1.145.24



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