L5 - Olympus ScanR+CellR

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|microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light
 
|microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light
 
|obj1=Olympus UPlanSApo 4x 0.16
 
|obj1=Olympus UPlanSApo 4x 0.16
|obj2=Olympus UPlanSApo 40X 0.9
+
|obj2=Olympus UPlanSApo 10x 0.4
 
|obj3=Olympus UPlanFluor 20X 0.5
 
|obj3=Olympus UPlanFluor 20X 0.5
|obj4=Olympus UPlanSApo 40X 0.9
+
|obj5=Olympus UPlanSApo 40X 0.9
 +
|obj6=Olympus UPlanFLN 40X 0.75 Ph2
 
|obj7=Olympus UPlanSApo 60x 1.35 Oil
 
|obj7=Olympus UPlanSApo 60x 1.35 Oil
 
|obj8=Olympus UPlanSApo 100x 1.4 Oil
 
|obj8=Olympus UPlanSApo 100x 1.4 Oil

Revision as of 12:09, 17 May 2010

Suitable for long-term observation of living cells, Fast multi-fluorescence 3-D time-lapse, Fast and precise image acquisition and analysis, robust automated object detection and cell separation, visual correlation of cell images and analysis results..
MPI24 .jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, U50)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Olympus - IX81, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light
objectives
Olympus UPlanSApo 4x 0.16
Olympus UPlanSApo 10x 0.4
Olympus UPlanFluor 20X 0.5
Olympus UPlanSApo 40X 0.9
Olympus UPlanFLN 40X 0.75 Ph2
Olympus UPlanSApo 60x 1.35 Oil
Olympus UPlanSApo 100x 1.4 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (Xenon)
detection Hamamatsu Camera ORCA ER, progressive scan interline CCD, 1344 x 1024 pixels, 6.45 um pixel size, 14.75 MHz digitization, 8 e- rms readout noise, 8 fps @ full frame, 18.000 e- full well capacity, 2250:1 dynamic range, peltier cooling (-20 degrees C @ 20 degrees C ambient), 12 bit ADC, 0.1 electron/pixel/sec dark current, up to 10x analog gain, offset functions
reflectors MT20 Excitation Unit

- EXCITATION FILTERS
387 / 403 / 485 / 492 / 560 / 572/ 650

- DICHROICS AND EMISSION FILTERS

* POSITION 1:
D 405-435/450-485/510-550/590-675
M 450-475/515-545/600-665

* POSITION 2:
D QBP 410-465, 504-538, 581-632, 669-743
M QBP 415-461, 507-533, 586-625, 673-722

* POSITION 3: EMPTY

* POSITION 4: EMPTY

* POSITION 5:
D 485-570/605-690
M 500-560/615-675

* POSITION 6
D 440-490/510-575/610-675
M 450-480/525-560/615-660

features Dual System. It can work as Scan R or as CELL R
SCAN R
Modular microscope-based imaging platform designed for fully automated image acquisition and data analysis of biological samples. scan^R can handle many different formats e.g. multi-well plates, slides or custom-built arrays. The unmatched flexibility and open design make it equally adept at routine and advanced applications. With its powerful analysis module for biological functional assays, it is the perfect tool for assay development and high-content screening. This provides complex image analysis and advanced data evaluation.
CELL R
consists of an all-in-one illumination system MT20, highly sensitive digital cameras and a hyper-precision hardware control board."Dual System. It can work as Scan R or as CELL R <br />SCAN R <br /> Modular microscope-based imaging platform designed for fully automated image acquisition and data analysis of biological samples. scan^R can handle many different formats e.g. multi-well plates, slides or custom-built arrays. The unmatched flexibility and open design make it equally adept at routine and advanced applications. With its powerful analysis module for biological functional assays, it is the perfect tool for assay development and high-content screening. This provides complex image analysis and advanced data evaluation. <br /> CELL R<br /> consists of an all-in-one illumination system MT20, highly sensitive digital cameras and a hyper-precision hardware control board." cannot be used as a page name in this wiki.
software -
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 405679



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