LSM 510, inverse, BIOTEC
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=BIO10 |
| − | |system-name= | + | |system-name=LSM 510, inverse, BIOTEC |
| − | |type= | + | |type=Not listed |
|category=Laser Scanning Confocal | |category=Laser Scanning Confocal | ||
| − | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
| − | |building= | + | |building=BIOTEC |
| − | |room= | + | |room=227 |
| − | | | + | |Facility-logo=Imaging@Biotec.jpg |
| − | | | + | |Institute-logo=Biotec logo.png |
|description=Our [[Zeiss LSM 510]] [[confocal]] laser scanning microscope is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | |description=Our [[Zeiss LSM 510]] [[confocal]] laser scanning microscope is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | ||
|applications=- confocal scanning microscopy | |applications=- confocal scanning microscopy | ||
| Line 14: | Line 14: | ||
|stand-name=Zeiss - Axiovert 200M | |stand-name=Zeiss - Axiovert 200M | ||
|stand=Inverted | |stand=Inverted | ||
| − | |microscope= | + | |microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC |
|obj1=Zeiss EC Plan-Neofluar 10x 0.3 | |obj1=Zeiss EC Plan-Neofluar 10x 0.3 | ||
|obj2=Zeiss EC Plan-Neofluar 20x 0.5 | |obj2=Zeiss EC Plan-Neofluar 20x 0.5 | ||
| Line 28: | Line 28: | ||
|ill6=Laser HeNe 633 nm | |ill6=Laser HeNe 633 nm | ||
|detection=point scanner, 2 confocal PMTs, 1 META detector, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | |detection=point scanner, 2 confocal PMTs, 1 META detector, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
| − | |reflectors= | + | |reflectors=DAPI : EX 365/12 ; BS 395 ; EM LP 397<br>FITC : EX 470/40 ; BS 510 ; EM LP 515<br>TRITC : EX 546/12 ; BS 580 ; EM LP 590 LP |
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
|software=Zeiss AIM | |software=Zeiss AIM | ||
|incubation=Stage incubator (T) | |incubation=Stage incubator (T) | ||
| − | | | + | |link0=http://www.biotec.tu-dresden.de/fileadmin/technology/imaging/LSM_510_-_manual.pdf |
| + | |link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_power_:_how_to_measure_and_values | ||
|link2=- | |link2=- | ||
| − | |inv= | + | |inv=121528 |
|objectives=10x/0.3<br>20x/0.5<br>40x/0.95 corr<br>40x/1.2 W corr<br>100x/1.4 Oil DIC | |objectives=10x/0.3<br>20x/0.5<br>40x/0.95 corr<br>40x/1.2 W corr<br>100x/1.4 Oil DIC | ||
|illumination=HBO lamp<br>Halogen lamp<br>405 diode laser<br>458, 477(optional), 488, 514 argon laser<br>543 HeNe laser<br>633 HeNe laser | |illumination=HBO lamp<br>Halogen lamp<br>405 diode laser<br>458, 477(optional), 488, 514 argon laser<br>543 HeNe laser<br>633 HeNe laser | ||
}} | }} | ||
Latest revision as of 13:25, 23 October 2013
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[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Zeiss - Axiovert 200M, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination | |||
| detection | point scanner, 2 confocal PMTs, 1 META detector, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
| reflectors | DAPI : EX 365/12 ; BS 395 ; EM LP 397 FITC : EX 470/40 ; BS 510 ; EM LP 515 TRITC : EX 546/12 ; BS 580 ; EM LP 590 LP | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
| software | Zeiss AIM | ||
| incubation | Stage incubator (T) | ||
| links |
| ||
| inv.nr. | 121528 |
