LSM 700, inverse, BIOTEC

From BioDIP
(Difference between revisions)
Jump to: navigation, search
Line 11: Line 11:
 
|description=It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
|description=It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
|applications=2D images, z-stacks, time lapse, bleaching and tile scans
 
|applications=2D images, z-stacks, time lapse, bleaching and tile scans
 +
|image=Zeiss LSM 700.jpg
 
|stand-name=Zeiss - Axiovert 200M
 
|stand-name=Zeiss - Axiovert 200M
 
|stand=Inverted
 
|stand=Inverted
Line 28: Line 29:
 
FITC : EX 480/30 ; BS 505 ; EM 535/40 <br>
 
FITC : EX 480/30 ; BS 505 ; EM 535/40 <br>
 
DsRed : EX 545/10 ; BS 555 ; EM 600/70 <br>
 
DsRed : EX 545/10 ; BS 555 ; EM 600/70 <br>
GFP/RFP : EX  ; BS  ; EM  
+
GFP/RFP : EX  ; BS  ; EM
 
|features=-
 
|features=-
 
|software=ZEN 2010
 
|software=ZEN 2010

Revision as of 10:34, 6 April 2011

Suitable for 2D images, z-stacks, time lapse, bleaching and tile scans.
250px


Directions

Biotec logo.png
Imaging@Biotec.jpg Imaging@Biotec"Imaging@Biotec" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. (BIOTEC, 227)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axiovert 200M, Inverted stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss C-Apochromat 40x 1.2 W
illumination
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 555nm
Laser DPSS 639nm
Transmitted Light (Halogen)
Fluorescence (HBO)
detection 2 PMTs

1 transmission detector

reflectors Dapi : EX 350/50 ; BS 395 ; EM LP 420

FITC : EX 480/30 ; BS 505 ; EM 535/40
DsRed : EX 545/10 ; BS 555 ; EM 600/70
GFP/RFP : EX  ; BS  ; EM

features -
software ZEN 2010
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



Personal tools