LSM 780, upright, CRTD

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|Facility-logo=Imaging@Biotec.jpg
 
|Facility-logo=Imaging@Biotec.jpg
 
|Institute-logo=CRTD logo.PNG
 
|Institute-logo=CRTD logo.PNG
|description=It is an upright Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. A selection of objective lenses is listed below, but more are available, too.
+
|description=It is an upright Laser Scanning Confocal microscope with 32 spectral detection channels (GaAsP), 2 PMTs, one Airyscan-detector and one transmission PMT. A selection of objective lenses is listed below, but more are available, too.
|applications=the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.
+
|applications=the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, spectral unmixing of overlapping emission spectra, Airyscan super resolution. It can acquire brightfield and DIC images with the transmission detector.
 
|image=LSM 780, upright, CRTD.jpg
 
|image=LSM 780, upright, CRTD.jpg
 
|stand-name=Zeiss - Axio Imager
 
|stand-name=Zeiss - Axio Imager
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|detection=32 PMT channels (GaAsP)<br>
 
|detection=32 PMT channels (GaAsP)<br>
 
2 PMTs<br>
 
2 PMTs<br>
transmission detector
+
1 Airyscan (GaAsP)<br>
 +
transmission detector PMT
 
|reflectors=Zeiss set 49: DAPI<br>
 
|reflectors=Zeiss set 49: DAPI<br>
 
Zeiss set 38 Endow: GFP<br>
 
Zeiss set 38 Endow: GFP<br>
 
Zeiss set 43: Cy3
 
Zeiss set 43: Cy3
|features=spectral scan
+
|features=spectral scan, Airyscan
|software=Zen 2010
+
|software=Zen 2012
 
|incubation=Not available
 
|incubation=Not available
 
|inv=82537
 
|inv=82537
 
}}
 
}}

Revision as of 10:41, 18 March 2016

Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, spectral unmixing of overlapping emission spectra, Airyscan super resolution. It can acquire brightfield and DIC images with the transmission detector..
LSM 780, upright, CRTD.jpg


Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.123)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axio Imager, Upright stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss W N-Achroplan 20x/0.5
Zeiss C-Apochromat 10x 0.45 W
Zeiss C-Apochromat 40x 1.2 W
Zeiss alpha Plan-Apochromat 100x 1,46 oil
illumination
Laser Diode 405 nm
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection 32 PMT channels (GaAsP)

2 PMTs
1 Airyscan (GaAsP)
transmission detector PMT

reflectors Zeiss set 49: DAPI

Zeiss set 38 Endow: GFP
Zeiss set 43: Cy3

features spectral scan, Airyscan
software Zen 2012
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 82537



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