LSM 780, upright, CRTD
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=BIO12 |
| − | |system-name= | + | |system-name=LSM 780, upright, CRTD |
|type=Microscope | |type=Microscope | ||
|category=Laser Scanning Confocal | |category=Laser Scanning Confocal | ||
| − | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
|building=CRTD | |building=CRTD | ||
|room=-1.123 | |room=-1.123 | ||
|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
|Institute-logo=CRTD logo.PNG | |Institute-logo=CRTD logo.PNG | ||
| − | |description=It is an upright Laser Scanning Confocal microscope with | + | |description=It is an upright Laser Scanning Confocal microscope with 32 spectral detection channels (GaAsP), 2 PMTs, one Airyscan-detector and one transmission PMT. A selection of objective lenses is listed below, but more are available, too. |
| − | |applications=the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, | + | |applications=the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, spectral unmixing of overlapping emission spectra, Airyscan super resolution. It can acquire brightfield and DIC images with the transmission detector. |
| + | |image=LSM 780, upright, CRTD.jpg | ||
|stand-name=Zeiss - Axio Imager | |stand-name=Zeiss - Axio Imager | ||
|stand=Upright | |stand=Upright | ||
|microscope=- | |microscope=- | ||
|obj1=Zeiss Plan-Apochromat 10x 0.45 | |obj1=Zeiss Plan-Apochromat 10x 0.45 | ||
| − | |obj2=Zeiss Plan-Apochromat 20x 0.8 | + | |obj2=Zeiss C-Apochromat 10x 0.45 W |
| − | |obj4=Zeiss C-Apochromat | + | |obj3=Zeiss Plan-Apochromat 20x 0.8 |
| + | |obj4=Zeiss W N-Achroplan 20x/0.5 | ||
| + | |obj6=Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC | ||
| + | |obj7=Zeiss C-Apochromat 40x 1.2 W | ||
| + | |obj8=Zeiss W Plan-Apochromat 40x 1.0 | ||
| + | |obj9=Zeiss Plan-Apochromat 40x 1.4 oil | ||
| + | |obj10=Zeiss Alpha Plan-Apochromat 100x 1.46 Oil DIC | ||
|ill1=Laser Diode 405 nm | |ill1=Laser Diode 405 nm | ||
|ill2=Laser Argon Multiline 458, 488, 514 nm | |ill2=Laser Argon Multiline 458, 488, 514 nm | ||
| Line 26: | Line 33: | ||
|detection=32 PMT channels (GaAsP)<br> | |detection=32 PMT channels (GaAsP)<br> | ||
2 PMTs<br> | 2 PMTs<br> | ||
| − | transmission detector | + | 1 Airyscan (GaAsP)<br> |
| + | transmission detector PMT | ||
|reflectors=Zeiss set 49: DAPI<br> | |reflectors=Zeiss set 49: DAPI<br> | ||
Zeiss set 38 Endow: GFP<br> | Zeiss set 38 Endow: GFP<br> | ||
| − | Zeiss set 43: Cy3 | + | Zeiss set 43: Cy3 |
| − | + | |features=spectral scan, Airyscan | |
| − | + | |software=ZEN 2.1 SP2 version 13.0.1.522 | |
| − | |features=spectral scan | + | |
| − | |software= | + | |
|incubation=Not available | |incubation=Not available | ||
| − | |inv= | + | |inv=82537 |
}} | }} | ||
Latest revision as of 09:16, 20 February 2017
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Zeiss - Axio Imager, Upright stand, - | ||
| objectives |
| ||
| illumination | |||
| detection | 32 PMT channels (GaAsP) 2 PMTs | ||
| reflectors | Zeiss set 49: DAPI Zeiss set 38 Endow: GFP | ||
| features | spectral scan, Airyscan | ||
| software | ZEN 2.1 SP2 version 13.0.1.522 | ||
| incubation | Not available | ||
| links |
| ||
| inv.nr. | 82537 |
