LSM 780, upright, CRTD

From BioDIP
(Difference between revisions)
Jump to: navigation, search
Line 31: Line 31:
 
|reflectors=Zeiss set 49: DAPI<br>
 
|reflectors=Zeiss set 49: DAPI<br>
 
Zeiss set 38 Endow: GFP<br>
 
Zeiss set 38 Endow: GFP<br>
Zeiss set 43: Cy3<br>
+
Zeiss set 43: Cy3
Zeiss set 46: YFP<br>
+
 
Zeiss set 47: CFP HE
+
 
 
|features=spectral scan
 
|features=spectral scan
 
|software=Zen 2010
 
|software=Zen 2010

Revision as of 10:48, 11 January 2012

Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector..
[[image:|250px]]


Directions

CRTD logo.PNG
Imaging@Biotec.jpg Imaging@Biotec"Imaging@Biotec" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. (CRTD, -1.123)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axio Imager, Upright stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 1.4 oil
Zeiss C-Apochromat 63x 1.2 W
Zeiss alpha Plan-Apochromat 100x 1,46 oil
illumination
Laser Diode 405 nm
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection 32 PMT channels (GaAsP)

2 PMTs
transmission detector

reflectors Zeiss set 49: DAPI

Zeiss set 38 Endow: GFP
Zeiss set 43: Cy3

features spectral scan
software Zen 2010
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



Personal tools