LSM 780/FCS, inverse, BIOTEC
From BioDIP
(Difference between revisions)
| Line 7: | Line 7: | ||
|building=BIOTEC | |building=BIOTEC | ||
|room=221 | |room=221 | ||
| − | |description=It is an inverted Laser Scanning Confocal microscope with up to | + | |description=It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides F(C)CS functionality by the Confocr3 module. For FCS please ask Wolfgang Staroske. |
|applications=the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector. | |applications=the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector. | ||
|image=Zeiss LSM 780.jpg | |image=Zeiss LSM 780.jpg | ||
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|obj3=Zeiss Plan-Apochromat 40x 0,95 korr. | |obj3=Zeiss Plan-Apochromat 40x 0,95 korr. | ||
|obj4=Zeiss C-Apochromat 40x 1.2 W | |obj4=Zeiss C-Apochromat 40x 1.2 W | ||
| − | |obj5=Zeiss | + | |obj5=Zeiss C-Apochromat 63x 1.2 W |
| − | |obj6=Zeiss | + | |obj6=Zeiss Plan-Apochromat 63x 1.4 Oil |
|ill1=Laser Diode 405 nm | |ill1=Laser Diode 405 nm | ||
|ill2=Laser Argon Multiline 458, 488, 514 nm | |ill2=Laser Argon Multiline 458, 488, 514 nm | ||
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|ill6=Fluorescence (HBO) | |ill6=Fluorescence (HBO) | ||
|ill7=Transmitted Light (Halogen) | |ill7=Transmitted Light (Halogen) | ||
| − | |detection= | + | |detection=32 PMT channels (GaAsP) |
| + | 2 PMTs | ||
transmission detector | transmission detector | ||
| − | |||
|reflectors=Zeiss set 49: DAPI | |reflectors=Zeiss set 49: DAPI | ||
Zeiss set 38: GFP | Zeiss set 38: GFP | ||
Revision as of 18:18, 8 November 2010
Directions
[[image:|150px|text-bottom|right]]
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | Zeiss - Axio Observer, Inverted stand, - | ||
| objectives | |||
| illumination | |||
| detection | 32 PMT channels (GaAsP)
2 PMTs transmission detector | ||
| reflectors | Zeiss set 49: DAPI
Zeiss set 38: GFP Zeiss set 43: Cy3 Zeiss set 50: Cy5 | ||
| features | F(C)CS with ConfoCor 3 unit | ||
| software | Zen 2010 | ||
| incubation | Not available | ||
| links |
| ||
| inv.nr. | 0 |
