LSM 780/FCS, inverse, BIOTEC
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=BIO13 |
| − | |system-name= | + | |system-name=LSM 780/FCS, inverse, BIOTEC |
|type=Microscope | |type=Microscope | ||
|category=Laser Scanning Confocal | |category=Laser Scanning Confocal | ||
| − | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
|building=BIOTEC | |building=BIOTEC | ||
|room=221 | |room=221 | ||
|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
| − | |Institute-logo= | + | |Institute-logo=Biotec logo.png |
|description=It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides F(C)CS functionality by the ConfoCor3 module. For FCS please ask Wolfgang Staroske. | |description=It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides F(C)CS functionality by the ConfoCor3 module. For FCS please ask Wolfgang Staroske. | ||
| − | |applications=the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector | + | |applications=the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector |
|image=Zeiss LSM 780.jpg | |image=Zeiss LSM 780.jpg | ||
|stand-name=Zeiss - Axio Observer | |stand-name=Zeiss - Axio Observer | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=- | |microscope=- | ||
| − | |obj1=Zeiss | + | |obj1=Zeiss Plan-Apochromat 10x 0.45 |
| − | |obj2=Zeiss | + | |obj2=Zeiss Plan-Apochromat 20x 0.8 |
| − | + | ||
|obj4=Zeiss C-Apochromat 40x 1.2 W | |obj4=Zeiss C-Apochromat 40x 1.2 W | ||
| − | |||
|obj6=Zeiss Plan-Apochromat 63x 1.4 Oil | |obj6=Zeiss Plan-Apochromat 63x 1.4 Oil | ||
| + | |obj7=Zeiss Plan Neofluar 40x 1.3 oil | ||
|ill1=Laser Diode 405 nm | |ill1=Laser Diode 405 nm | ||
|ill2=Laser Argon Multiline 458, 488, 514 nm | |ill2=Laser Argon Multiline 458, 488, 514 nm | ||
| Line 27: | Line 26: | ||
|ill5=Fluorescence (Metal Halide, HXP, 120W) | |ill5=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill6=Transmitted Light (Halogen) | |ill6=Transmitted Light (Halogen) | ||
| − | |detection=32 PMT channels (GaAsP) | + | |detection=32 PMT channels (GaAsP)<br> |
| − | 2 PMTs | + | 2 PMTs<br> |
| − | transmission detector | + | transmission detector<br> |
| − | |reflectors=Zeiss set 49: DAPI | + | 2 APDs (Confocor 3) |
| − | Zeiss set 38: GFP | + | |reflectors=Zeiss set 49: DAPI<br> |
| − | Zeiss set 43: Cy3 | + | Zeiss set 38: GFP<br> |
| − | + | Zeiss set 43: Cy3<br> | |
| − | |features=F(C)CS with ConfoCor 3 unit | + | DIC/POL |
| − | |software=Zen | + | |features=spectral scan, F(C)CS with ConfoCor 3 unit |
| − | |incubation= | + | |software=Zen 2011 SP3 |
| + | |incubation=Cage incubator (T+CO2) | ||
| + | |link0=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/LSM780_-_start_up_%26_shut_down | ||
|link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_Power_LSM780 | |link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_Power_LSM780 | ||
| − | |inv= | + | |inv=184202 |
}} | }} | ||
Latest revision as of 13:53, 17 October 2014
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Zeiss - Axio Observer, Inverted stand, - | ||
| objectives | |||
| illumination | |||
| detection | 32 PMT channels (GaAsP) 2 PMTs | ||
| reflectors | Zeiss set 49: DAPI Zeiss set 38: GFP | ||
| features | spectral scan, F(C)CS with ConfoCor 3 unit | ||
| software | Zen 2011 SP3 | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | 184202 |
