LSM 780/FCS, inverse, BIOTEC

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Line 15: Line 15:
 
|stand=Inverted
 
|stand=Inverted
 
|microscope=-
 
|microscope=-
|obj1=Zeiss alpha Plan-Apochromat 100x 1.46 Oil DIC
+
|obj1=Zeiss Plan-Apochromat 10x 0.45
 
|obj2=Zeiss Plan-Apochromat 20x 0.8
 
|obj2=Zeiss Plan-Apochromat 20x 0.8
|obj3=Zeiss Plan-Apochromat 40x 0,95 korr.
 
 
|obj4=Zeiss C-Apochromat 40x 1.2 W
 
|obj4=Zeiss C-Apochromat 40x 1.2 W
|obj5=Zeiss C-Apochromat 63x 1.2 W
 
 
|obj6=Zeiss Plan-Apochromat 63x 1.4 Oil
 
|obj6=Zeiss Plan-Apochromat 63x 1.4 Oil
 
|obj7=Zeiss Plan Neofluar 40x 1.3 oil
 
|obj7=Zeiss Plan Neofluar 40x 1.3 oil

Latest revision as of 13:53, 17 October 2014

Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.
Zeiss LSM 780.jpg


[edit] Directions

Biotec logo.png
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 221)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Zeiss - Axio Observer, Inverted stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss C-Apochromat 40x 1.2 W
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss Plan Neofluar 40x 1.3 oil
illumination
Laser Diode 405 nm
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection 32 PMT channels (GaAsP)

2 PMTs
transmission detector
2 APDs (Confocor 3)

reflectors Zeiss set 49: DAPI

Zeiss set 38: GFP
Zeiss set 43: Cy3
DIC/POL

features spectral scan, F(C)CS with ConfoCor 3 unit
software Zen 2011 SP3
incubation Cage incubator (T+CO2)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. 184202



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