LSM 780/FCS, inverse, BIOTEC

Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.

Directions

Imaging@BIOTEC/CRTD (BIOTEC, 221)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope	Zeiss - Axio Observer, Inverted stand, -
objectives	Zeiss Plan-Apochromat 20x 0.8 Zeiss Plan-Apochromat 40x 0,95 korr. Zeiss C-Apochromat 40x 1.2 W Zeiss C-Apochromat 63x 1.2 W Zeiss Plan-Apochromat 63x 1.4 Oil
illumination	Laser Diode 405 nm Laser Argon Multiline 458, 488, 514 nm Laser DPSS 561 nm Laser HeNe 633 nm Fluorescence (Metal Halide, HXP, 120W) Transmitted Light (Halogen)
detection	32 PMT channels (GaAsP) 2 PMTs transmission detector
reflectors	Zeiss set 49: DAPI Zeiss set 38: GFP Zeiss set 43: Cy3 Zeiss set 50: Cy5
features	spectral scan, F(C)CS with ConfoCor 3 unit
software	Zen 2010
incubation	Cage incubator (T+CO2)
links	manual laser power values [ beam path] [ advanced system check]
inv.nr.	184202

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