LSM 780/FLIM, inverse, CRTD
From BioDIP
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=BIO14 |
|system-name=LSM 780/FLIM, inverse, CRTD | |system-name=LSM 780/FLIM, inverse, CRTD | ||
|type=Microscope | |type=Microscope | ||
|category=Laser Scanning Confocal | |category=Laser Scanning Confocal | ||
| − | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
|building=CRTD | |building=CRTD | ||
|room=-1.124 | |room=-1.124 | ||
| Line 10: | Line 10: | ||
|Institute-logo=CRTD logo.PNG | |Institute-logo=CRTD logo.PNG | ||
|description=It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides FLIM functionality. | |description=It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides FLIM functionality. | ||
| − | |applications= | + | |applications=the detection of multiple fluorescent dyes (but no DAPI), FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector |
| + | |image=LSM780FLIM inverse CRTD.jpg | ||
|stand-name=Zeiss - Axio Observer | |stand-name=Zeiss - Axio Observer | ||
|stand=Inverted | |stand=Inverted | ||
| Line 17: | Line 18: | ||
|obj2=Zeiss Plan-Apochromat 20x 0.8 | |obj2=Zeiss Plan-Apochromat 20x 0.8 | ||
|obj3=Zeiss C-Apochromat 40x 1.2 W | |obj3=Zeiss C-Apochromat 40x 1.2 W | ||
| − | | | + | |obj5=Zeiss alpha Plan-Apochromat 63x 1.46 Oil |
|ill1=Laser Diode 440 nm (pulsed) | |ill1=Laser Diode 440 nm (pulsed) | ||
|ill2=Laser Diode 475 nm (pulsed) | |ill2=Laser Diode 475 nm (pulsed) | ||
| Line 30: | Line 31: | ||
|reflectors=Zeiss set 49: DAPI <br> | |reflectors=Zeiss set 49: DAPI <br> | ||
Zeiss set 38: GFP <br> | Zeiss set 38: GFP <br> | ||
| + | Zeiss set 47: CFP <br> | ||
| + | Zeiss set 46: YFP <br> | ||
Zeiss set 43: Cy3 | Zeiss set 43: Cy3 | ||
| − | |features=spectral scan, FLIM | + | |features=spectral scan, FLIM dual channel unit from B&H |
| − | |software=ZEN 2011 | + | |software=ZEN black 2011 |
| − | |incubation=Cage incubator (T+CO2) | + | |incubation=Cage incubator (T+CO2+Humidity) |
| − | |inv= | + | |link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_Power_LSM780/FLIM |
| + | |inv=84844 | ||
}} | }} | ||
Latest revision as of 13:54, 17 October 2014
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | Zeiss - Axio Observer, Inverted stand, - | ||
| objectives | |||
| illumination | |||
| detection | 32 PMT channels (GaAsP) 2 PMTs | ||
| reflectors | Zeiss set 49: DAPI Zeiss set 38: GFP | ||
| features | spectral scan, FLIM dual channel unit from B&H | ||
| software | ZEN black 2011 | ||
| incubation | Cage incubator (T+CO2+Humidity) | ||
| links |
| ||
| inv.nr. | 84844 |
