LSM 780/FLIM, inverse, CRTD

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|Institute-logo=CRTD logo.PNG
 
|Institute-logo=CRTD logo.PNG
 
|description=It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides FLIM functionality.
 
|description=It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides FLIM functionality.
−
|applications=Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.
+
|applications=Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector
 
|stand-name=Zeiss - Axio Observer
 
|stand-name=Zeiss - Axio Observer
 
|stand=Inverted
 
|stand=Inverted
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Zeiss set 38: GFP <br>
 
Zeiss set 38: GFP <br>
 
Zeiss set 43: Cy3
 
Zeiss set 43: Cy3
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|features=spectral scan, FLIM (B&H) unit  
+
|features=spectral scan, FLIM (B&H) unit
 
|software=ZEN 2011
 
|software=ZEN 2011
 
|incubation=Cage incubator (T+CO2)
 
|incubation=Cage incubator (T+CO2)
 
|inv=0
 
|inv=0
 
}}
 
}}

Revision as of 16:32, 9 October 2012

Suitable for Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.
[[image:|250px]]


Directions

CRTD logo.PNG
Imaging@Biotec.jpg Imaging@Biotec"Imaging@Biotec" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. (CRTD, -1.124)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axio Observer, Inverted stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss C-Apochromat 40x 1.2 W
Zeiss alpha Plan-Apochromat 100x 1,46 oil
illumination
detection 32 PMT channels (GaAsP)

2 PMTs
transmission detector

reflectors Zeiss set 49: DAPI

Zeiss set 38: GFP
Zeiss set 43: Cy3

features spectral scan, FLIM (B&H) unit
software ZEN 2011
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0


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