LSM 780/FLIM, inverse, CRTD

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|Institute-logo=CRTD logo.PNG
 
|Institute-logo=CRTD logo.PNG
 
|description=It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides FLIM functionality.
 
|description=It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides FLIM functionality.
|applications=Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector
+
|applications=the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector
 
|stand-name=Zeiss - Axio Observer
 
|stand-name=Zeiss - Axio Observer
 
|stand=Inverted
 
|stand=Inverted

Revision as of 13:05, 18 January 2013

Suitable for the detection of multiple fluorescent dyes, FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.
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Directions

CRTD logo.PNG
Imaging@Biotec.jpg Imaging@Biotec"Imaging@Biotec" is not in the list of possible values (DZNE - Imaging Platform, EM- and Histo-Facility CRTD / BIOTEC, Image Processing@BIOTEC, Imaging@BIOTEC/CRTD, MPI-CBG LMF, MPI-CBG LMF - Quantitative Image Analysis Unit, MPI-CBG TDS, MTZ Imaging, MPI-CBG EM, BioDIP) for this property. (CRTD, -1.124)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axio Observer, Inverted stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss C-Apochromat 40x 1.2 W
Zeiss alpha Plan-Apochromat 100x 1,46 oil
illumination
Laser Diode 440 nm (pulsed)
Laser Diode 475 nm (pulsed)
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection 32 PMT channels (GaAsP)

2 PMTs
transmission detector

reflectors Zeiss set 49: DAPI

Zeiss set 38: GFP
Zeiss set 43: Cy3

features spectral scan, FLIM dual channel unit from B&H
software ZEN 2011
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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