LSM 780/FLIM, inverse, CRTD

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{{Equipment
 
{{Equipment
−
|system-number=BIO16
+
|system-number=BIO14
 
|system-name=LSM 780/FLIM, inverse, CRTD
 
|system-name=LSM 780/FLIM, inverse, CRTD
 
|type=Microscope
 
|type=Microscope

Revision as of 15:21, 27 May 2013

Suitable for the detection of multiple fluorescent dyes (but no DAPI), FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.
LSM780FLIM inverse CRTD.jpg


Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.124)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axio Observer, Inverted stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss C-Apochromat 40x 1.2 W
Zeiss alpha Plan-Apochromat 100x 1,46 oil
illumination
Laser Diode 440 nm (pulsed)
Laser Diode 475 nm (pulsed)
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection 32 PMT channels (GaAsP)

2 PMTs
transmission detector

reflectors Zeiss set 49: DAPI

Zeiss set 38: GFP
Zeiss set 43: Cy3

features spectral scan, FLIM dual channel unit from B&H
software ZEN 2011
incubation Cage incubator (T+CO2+Humidity)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. 84844


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