LZ1 - Zeiss Lightsheet Z.1
From BioDIP
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{{Equipment | {{Equipment | ||
| − | |system-number=MPI- | + | |system-number=MPI- LZ1 |
|system-name=ZEISS LightSheet LZ.1 | |system-name=ZEISS LightSheet LZ.1 | ||
|type=Microscope | |type=Microscope | ||
| Line 6: | Line 6: | ||
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
| − | |room= | + | |room=356 |
|Facility-logo=MPI-CBG-Lmf-logo-big.png | |Facility-logo=MPI-CBG-Lmf-logo-big.png | ||
|Institute-logo=Logo MPI-CBG.jpg | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions. | |description=The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions. | ||
|applications=Live imaging in 3D and 4D | |applications=Live imaging in 3D and 4D | ||
| − | |image= | + | |image=LZ1 - Zeiss Lightsheet Z.1.jpg |
|stand-name=Zeiss - LightSheet LZ1 Stand | |stand-name=Zeiss - LightSheet LZ1 Stand | ||
|stand=3D | |stand=3D | ||
|microscope=- | |microscope=- | ||
| + | |obj1=Zeiss Plan Apochromat 20x 1.0 W DIC | ||
| + | |obj2=Zeiss Plan Apochromat 20x 1.0 W DIC | ||
| + | |obj3=Zeiss EC Plan Neofluar 5x 0.16 | ||
| + | |obj4=Zeiss Plan Apochromat 40x 1.0 W DIC | ||
| + | |obj5=Zeiss Plan Apochromat 63x 1.0 W | ||
| + | |obj6=Zeiss Illumination Optics Lightsheet 10x 0.2 | ||
| + | |obj7=Zeiss Illumination Optics Lightsheet 10x 0.2 | ||
| + | |obj8=Zeiss Illumination Optics Lightsheet 5x 0.1 | ||
| + | |obj9=Zeiss Illumination Optics Lightsheet 5x 0.1 | ||
| + | |obj10=Zeiss Illumination Optics Lightsheet 10x 0.2 | ||
| + | |ill1=Laser Diode 405 nm | ||
| + | |ill2=Laser DPSS 488 nm | ||
| + | |ill3=Laser DPSS 561 nm | ||
| + | |ill4=Laser DPSS 639nm | ||
| + | |ill5=Transmitted Light (LED) | ||
|detection=Two sCMOS cameras; pixel size: 6.5 µm | |detection=Two sCMOS cameras; pixel size: 6.5 µm | ||
|reflectors=Laser blockers:<br>405/488/561<br>405/488/561/640<br>405/488/640<br>ND4 (RGB)<br> | |reflectors=Laser blockers:<br>405/488/561<br>405/488/561/640<br>405/488/640<br>ND4 (RGB)<br> | ||
| Line 25: | Line 40: | ||
|features=The system is equipped with a telescopic lens, which allows to zoom out up to 0.36x, and to zoom in up to 2.5x with any detection lens. | |features=The system is equipped with a telescopic lens, which allows to zoom out up to 0.36x, and to zoom in up to 2.5x with any detection lens. | ||
|software=ZEN Imaging Software 2012 (Black edition)<br>LightSheet Z.1 Multiview Processing<br>3D VisArt<br>Deconvolution | |software=ZEN Imaging Software 2012 (Black edition)<br>LightSheet Z.1 Multiview Processing<br>3D VisArt<br>Deconvolution | ||
| − | |incubation= | + | |incubation=Cage incubator (T+CO2) |
|inv=MOZG04089700000 | |inv=MOZG04089700000 | ||
}} | }} | ||
Latest revision as of 17:32, 3 February 2016
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[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Zeiss - LightSheet LZ1 Stand, 3D stand, - | ||
| objectives |
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| illumination | |||
| detection | Two sCMOS cameras; pixel size: 6.5 µm | ||
| reflectors | Laser blockers: 405/488/561 405/488/561/640 405/488/640 ND4 (RGB) Secondary beam splitters and associated emission filters: | ||
| features | The system is equipped with a telescopic lens, which allows to zoom out up to 0.36x, and to zoom in up to 2.5x with any detection lens. | ||
| software | ZEN Imaging Software 2012 (Black edition) LightSheet Z.1 Multiview Processing 3D VisArt Deconvolution"ZEN Imaging Software 2012 (Black edition)<br />LightSheet Z.1 Multiview Processing<br />3D VisArt<br />Deconvolution" cannot be used as a page name in this wiki. | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
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| inv.nr. | MOZG04089700000 |
