View source for LZ2 - Zeiss Lightsheet Z.1

{{Equipment
|system-number=MPI- LZ2
|system-name=ZEISS LightSheet LZ.1
|type=Microscope
|category=Single Plane Illumination
|facility=MPI-CBG LMF
|building=MPI-CBG
|room=356
|Facility-logo=MPI-CBG-Lmf-logo-big.png
|Institute-logo=Logo MPI-CBG.jpg
|description=The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions.
|applications=Live imaging of larger specimen in 3D and 4D<br>
imaging of larger cleared samples
|image=LZ1 - Zeiss Lightsheet Z.1.jpg
|stand-name=Zeiss - LightSheet LZ1 Stand
|stand=3D
|microscope=-
|obj1=Zeiss Plan Apochromat 20x 1.0 W DIC
|obj2=Zeiss Plan Apochromat 20x 1.0 W DIC
|obj3=Zeiss EC Plan Neofluar 5x 0.16
|obj4=Zeiss Plan Apochromat 40x 1.0 W DIC
|obj5=Zeiss Plan Apochromat 63x 1.0 W
|obj6=Zeiss Illumination Optics Lightsheet 10x 0.2
|obj7=Zeiss Illumination Optics Lightsheet 10x 0.2
|obj8=Zeiss Illumination Optics Lightsheet  5x 0.1
|obj9=Zeiss Illumination Optics Lightsheet 5x 0.1
|obj10=Zeiss Illumination Optics Lightsheet 10x 0.2
|ill1=Laser Diode 405 nm
|ill2=Laser DPSS 445 nm
|ill3=Laser DPSS 488 nm
|ill4=Laser DPSS 514 nm
|ill5=Laser DPSS 561 nm
|ill6=Laser DPSS 639nm
|ill7=Transmitted Light (LED)
|detection=Two sCMOS cameras; pixel size: 6.5 µm
|reflectors=Laser blockers:<br>405/488/561<br>405/488/561/640<br>405/488/640<br>ND4 (RGB)<br>
Secondary beam splitters and associated emission filters:<br>
LP 490 coupled to SP 490, and LP505<br>
LP 510 coupled to BP 420-470, and BP 525-565<br>
LP 560 coupled to BP 505-545, and BP 575-615<br>
LP 580 coupled to BP 525-565, and LP 585<br>
LP 640 coupled to BP 575-615, and LP 660<br>
|features=The system is equipped with a telescopic lens, which allows to zoom out up to 0.36x, and to zoom in up to 2.5x with any detection lens.
|software=ZEN Imaging Software 2012 (Black edition)<br>LightSheet Z.1 Multiview Processing<br>3D VisArt<br>Deconvolution
|incubation=Cage incubator (T+CO2)
}}