LZ2 - Zeiss Lightsheet Z.1
From BioDIP
(Difference between revisions)
(creation of page, copy whole page content from LZ1) |
|||
(7 intermediate revisions by one user not shown) | |||
Line 1: | Line 1: | ||
{{Equipment | {{Equipment | ||
− | |system-number=MPI-LZ2 | + | |system-number=MPI- LZ2 |
|system-name=ZEISS LightSheet LZ.1 | |system-name=ZEISS LightSheet LZ.1 | ||
|type=Microscope | |type=Microscope | ||
Line 10: | Line 10: | ||
|Institute-logo=Logo MPI-CBG.jpg | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions. | |description=The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions. | ||
− | |applications=Live imaging in 3D and 4D | + | |applications=Live imaging of larger specimen in 3D and 4D<br> |
+ | imaging of larger cleared samples | ||
|image=LZ1 - Zeiss Lightsheet Z.1.jpg | |image=LZ1 - Zeiss Lightsheet Z.1.jpg | ||
|stand-name=Zeiss - LightSheet LZ1 Stand | |stand-name=Zeiss - LightSheet LZ1 Stand | ||
Line 26: | Line 27: | ||
|obj10=Zeiss Illumination Optics Lightsheet 10x 0.2 | |obj10=Zeiss Illumination Optics Lightsheet 10x 0.2 | ||
|ill1=Laser Diode 405 nm | |ill1=Laser Diode 405 nm | ||
− | |ill2=Laser DPSS | + | |ill2=Laser DPSS 445 nm |
− | |ill3=Laser DPSS | + | |ill3=Laser DPSS 488 nm |
− | |ill4=Laser DPSS | + | |ill4=Laser DPSS 514 nm |
− | |ill5=Transmitted Light (LED) | + | |ill5=Laser DPSS 561 nm |
+ | |ill6=Laser DPSS 639nm | ||
+ | |ill7=Transmitted Light (LED) | ||
|detection=Two sCMOS cameras; pixel size: 6.5 µm | |detection=Two sCMOS cameras; pixel size: 6.5 µm | ||
|reflectors=Laser blockers:<br>405/488/561<br>405/488/561/640<br>405/488/640<br>ND4 (RGB)<br> | |reflectors=Laser blockers:<br>405/488/561<br>405/488/561/640<br>405/488/640<br>ND4 (RGB)<br> | ||
Line 41: | Line 44: | ||
|software=ZEN Imaging Software 2012 (Black edition)<br>LightSheet Z.1 Multiview Processing<br>3D VisArt<br>Deconvolution | |software=ZEN Imaging Software 2012 (Black edition)<br>LightSheet Z.1 Multiview Processing<br>3D VisArt<br>Deconvolution | ||
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
− | |||
}} | }} |
Latest revision as of 17:34, 3 February 2016
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
microscope | Zeiss - LightSheet LZ1 Stand, 3D stand, - | ||
objectives |
| ||
illumination | |||
detection | Two sCMOS cameras; pixel size: 6.5 µm | ||
reflectors | Laser blockers: 405/488/561 405/488/561/640 405/488/640 ND4 (RGB) Secondary beam splitters and associated emission filters: | ||
features | The system is equipped with a telescopic lens, which allows to zoom out up to 0.36x, and to zoom in up to 2.5x with any detection lens. | ||
software | ZEN Imaging Software 2012 (Black edition) LightSheet Z.1 Multiview Processing 3D VisArt Deconvolution"ZEN Imaging Software 2012 (Black edition)<br />LightSheet Z.1 Multiview Processing<br />3D VisArt<br />Deconvolution" cannot be used as a page name in this wiki. | ||
incubation | Cage incubator (T+CO2) | ||
links |
| ||
inv.nr. |