Light Microscopy for You
From BioDIP
(Difference between revisions)
(list of stuff that peopek need to know about light microscopy before they die (umm start working in lmf)) |
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| Line 1: | Line 1: | ||
| − | # Stokes Shift | + | # Admin intro |
| + | |||
| + | # What is contrast? (No light = no image; no contrast = no resolution) | ||
| + | # Fluorescence and Stokes Shift | ||
# Pixel Spacing (size?) Physical CCD dexels vs Picture Elements | # Pixel Spacing (size?) Physical CCD dexels vs Picture Elements | ||
# Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA | # Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA | ||
| Line 5: | Line 8: | ||
# Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01) | # Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01) | ||
# Point Spread Function / Airy Disk | # Point Spread Function / Airy Disk | ||
| + | # lens cleaning | ||
| + | # safety ; mercury lamps, lasers (jewelry, interlocks) | ||
Revision as of 09:52, 3 September 2010
- Admin intro
- What is contrast? (No light = no image; no contrast = no resolution)
- Fluorescence and Stokes Shift
- Pixel Spacing (size?) Physical CCD dexels vs Picture Elements
- Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
- What is confocal
- Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
- Point Spread Function / Airy Disk
- lens cleaning
- safety ; mercury lamps, lasers (jewelry, interlocks)
