Light Microscopy for You

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(add link for new user checklist and lots of content)
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# Admin intro
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=1 slide per point=
  
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# What is a Microscope - Provdides  Magnification and more importantly Resolution. 
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## Basic Ray Optics of a Lens.
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## refraction
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## Conjugate planes
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###Single Lens has 2 focal planes FFP and BFP
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### little microscope
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### What is confocal
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# Resolution
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## optical resolution  - Point Spread Function / Airy Disk
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##Pixel Spacing (size?) Physical CCD dexels vs Picture Elements vs point scanning
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## Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
 
# What is contrast? (No light = no image; no contrast = no resolution)
 
# What is contrast? (No light = no image; no contrast = no resolution)
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# Fluorescence and Stokes Shift
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## Fluorescence and Stokes Shift
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# Pixel Spacing (size?) Physical CCD dexels vs Picture Elements
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### Spectrum databases / viewers for planning expt.
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# Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
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# Detectors: CCD vs. PMT  Widefield vs point detection
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# What is confocal
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## Digitization
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# Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
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## Detector Saturation
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# Point Spread Function / Airy Disk
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# Objective language / reading
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# lens cleaning
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## Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
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## Lens Immersion media
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## old oil,  wrong oil, wrong collar setting
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## Lens cleaning
 
# safety ; mercury lamps, lasers (jewelry, interlocks)
 
# safety ; mercury lamps, lasers (jewelry, interlocks)
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# Project Planning / Workflow
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# Admin intro
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## New User 30 min [[Checklist_for_introducing_new_users_to_the_MPI-CBG_LMF/IPF_facility|new user checklist]]

Revision as of 11:41, 20 September 2010

1 slide per point

  1. What is a Microscope - Provdides Magnification and more importantly Resolution.
    1. Basic Ray Optics of a Lens.
    2. refraction
    3. Conjugate planes
      1. Single Lens has 2 focal planes FFP and BFP
      2. little microscope
      3. What is confocal
  2. Resolution
    1. optical resolution - Point Spread Function / Airy Disk
    2. Pixel Spacing (size?) Physical CCD dexels vs Picture Elements vs point scanning
    3. Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
  3. What is contrast? (No light = no image; no contrast = no resolution)
    1. Fluorescence and Stokes Shift
      1. Spectrum databases / viewers for planning expt.
  4. Detectors: CCD vs. PMT Widefield vs point detection
    1. Digitization
    2. Detector Saturation
  5. Objective language / reading
    1. Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
    2. Lens Immersion media
    3. old oil, wrong oil, wrong collar setting
    4. Lens cleaning
  6. safety ; mercury lamps, lasers (jewelry, interlocks)
  7. Project Planning / Workflow
  1. Admin intro
    1. New User 30 min new user checklist
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