Light Microscopy for You
From BioDIP
(Difference between revisions)
(add abstract beginning) |
|||
| Line 2: | Line 2: | ||
its not a course OK?! | its not a course OK?! | ||
What you need to know about light microscopy and the LMF/IPF but never knew to ask. | What you need to know about light microscopy and the LMF/IPF but never knew to ask. | ||
| + | Concepts you need to know / refresh, including tips and tricks, | ||
| + | concerning how to improve your imaging experiments and impress your RGL/PI, and TAC. | ||
| − | + | Target Audience: | |
| + | Everyone doing light microscopy- ESPECIALLY NEW-COMERS!!! | ||
| + | *1 slide per point | ||
| + | |||
| + | |||
| + | ==Content== | ||
| + | |||
| + | === Introduce yourself=== | ||
| + | # Who are we? | ||
| + | # Who are you? | ||
| + | |||
| + | ===Practical Microscopy Tips and Tricks=== | ||
# What is a Microscope - Provdides Magnification and more importantly Resolution. | # What is a Microscope - Provdides Magnification and more importantly Resolution. | ||
## Basic Ray Optics of a Lens. | ## Basic Ray Optics of a Lens. | ||
| Line 30: | Line 43: | ||
# Project Planning / Workflow | # Project Planning / Workflow | ||
| + | ===Admin=== | ||
# Admin intro | # Admin intro | ||
## New User 30 min [[Checklist_for_introducing_new_users_to_the_MPI-CBG_LMF/IPF_facility|new user checklist]] | ## New User 30 min [[Checklist_for_introducing_new_users_to_the_MPI-CBG_LMF/IPF_facility|new user checklist]] | ||
Revision as of 14:07, 20 September 2010
Contents |
Abstract here
its not a course OK?! What you need to know about light microscopy and the LMF/IPF but never knew to ask. Concepts you need to know / refresh, including tips and tricks, concerning how to improve your imaging experiments and impress your RGL/PI, and TAC.
Target Audience: Everyone doing light microscopy- ESPECIALLY NEW-COMERS!!!
- 1 slide per point
Content
Introduce yourself
- Who are we?
- Who are you?
Practical Microscopy Tips and Tricks
- What is a Microscope - Provdides Magnification and more importantly Resolution.
- Basic Ray Optics of a Lens.
- refraction
- Conjugate planes
- Single Lens has 2 focal planes FFP and BFP
- little microscope
- What is confocal
- Resolution
- optical resolution - Point Spread Function / Airy Disk
- Pixel Spacing (size?) Physical CCD dexels vs Picture Elements vs point scanning
- Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
- What is contrast? (No light = no image; no contrast = no resolution)
- Fluorescence and Stokes Shift
- Spectrum databases / viewers for planning expt.
- Fluorescence and Stokes Shift
- Detectors: CCD vs. PMT Widefield vs point detection
- Digitization
- Detector Saturation
- Objective language / reading
- Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
- Lens Immersion media
- old oil, wrong oil, wrong collar setting
- Lens cleaning
- safety ; mercury lamps, lasers (jewelry, interlocks)
- Project Planning / Workflow
Admin
- Admin intro
- New User 30 min new user checklist
