Light Microscopy for You

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==What is LM4U? - Briefing Abstract==
 
==What is LM4U? - Briefing Abstract==
−
Look, its not a course! OK?!
+
Look, its not really a taught course! OK?! Its a briefing for new users and whoever else needs to know the bare essentials of light microscopy.
−
This brief, crash course, covers:
+
This is not a substitute for a full "Basics of light microscopy course", but it will get you started.
−
#What you need to know about light microscopy and the LMF/IPF but never knew to ask.
+
This very brief, "crash course in light microscopy basics", covers:
−
#Concepts you need to know / refresh, including tips and tricks, concerning how to improve your imaging experiments and impress your RGL/PI, and TAC.
+
#What you need to know about light microscopy and the LMF/IPF, but never knew to ask.
−
#Safety: Lasers, mercury lamps, etc.  
+
##Concepts you need to know / refresh, including tips and tricks, concerning how to improve your imaging experiments and impress your RGL/PI, and TAC.
−
#How to use the LMF / IPF
+
##Safety: Lasers, mercury lamps, etc.  
 +
##How to use the LMF / IPF
  
−
Target Audience:
+
==Target Audience:==
 
Everyone doing light microscopy-  ESPECIALLY NEW-COMERS!!!
 
Everyone doing light microscopy-  ESPECIALLY NEW-COMERS!!!
  
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## old oil,  wrong oil, wrong collar setting
 
## old oil,  wrong oil, wrong collar setting
 
## Lens cleaning
 
## Lens cleaning
−
# safety ; mercury lamps, lasers (jewelry, interlocks)
 
 
# Project Planning / Workflow
 
# Project Planning / Workflow
 +
 +
===Safety===
 +
# mercury HBO lamps - explosion risk - what to do it it explodes.
 +
# lasers (jewelry, interlocks, eye level above laser level, dont disable/defeat laser safety devices, dont look at a laser with your remaining good eye. )
 +
# White light sources (HBO, MetalHalide etc. and Halogen) can also hurt your eyes.
  
 
===Admin===
 
===Admin===
 
# Admin intro
 
# Admin intro
 
## New User 30 min [[Checklist_for_introducing_new_users_to_the_MPI-CBG_LMF/IPF_facility|new user checklist]]
 
## New User 30 min [[Checklist_for_introducing_new_users_to_the_MPI-CBG_LMF/IPF_facility|new user checklist]]

Revision as of 12:03, 27 September 2010

Contents

LM4U - light microscopy, for you!

What is LM4U? - Briefing Abstract

Look, its not really a taught course! OK?! Its a briefing for new users and whoever else needs to know the bare essentials of light microscopy. This is not a substitute for a full "Basics of light microscopy course", but it will get you started. This very brief, "crash course in light microscopy basics", covers:

  1. What you need to know about light microscopy and the LMF/IPF, but never knew to ask.
    1. Concepts you need to know / refresh, including tips and tricks, concerning how to improve your imaging experiments and impress your RGL/PI, and TAC.
    2. Safety: Lasers, mercury lamps, etc.
    3. How to use the LMF / IPF

Target Audience:

Everyone doing light microscopy- ESPECIALLY NEW-COMERS!!!


Content

(1 slide per point)

Introduce yourself

  1. Who are we?
  2. Who are you?

Practical Microscopy Tips and Tricks

  1. What is a Microscope - Provdides Magnification and more importantly Resolution.
    1. Basic Ray Optics of a Lens.
    2. refraction
    3. Conjugate planes
      1. Single Lens has 2 focal planes FFP and BFP
      2. little microscope
      3. What is confocal
  2. Resolution
    1. optical resolution - Point Spread Function / Airy Disk
    2. Pixel Spacing (size?) Physical CCD dexels vs Picture Elements vs point scanning
    3. Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
  3. What is contrast? (No light = no image; no contrast = no resolution)
    1. Fluorescence and Stokes Shift
      1. Spectrum databases / viewers for planning expt.
  4. Detectors: CCD vs. PMT Widefield vs point detection
    1. Digitization
    2. Detector Saturation
  5. Objective language / reading
    1. Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
    2. Lens Immersion media
    3. old oil, wrong oil, wrong collar setting
    4. Lens cleaning
  6. Project Planning / Workflow

Safety

  1. mercury HBO lamps - explosion risk - what to do it it explodes.
  2. lasers (jewelry, interlocks, eye level above laser level, dont disable/defeat laser safety devices, dont look at a laser with your remaining good eye. )
  3. White light sources (HBO, MetalHalide etc. and Halogen) can also hurt your eyes.

Admin

  1. Admin intro
    1. New User 30 min new user checklist
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