Light Microscopy for You

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  1. Stokes Shift
  2. Pixel Spacing (size?) Physical CCD dexels vs Picture Elements
  3. Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
  4. What is confocal
  5. Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
  6. Point Spread Function / Airy Disk
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