Light Microscopy for You

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  1. Admin intro
  1. What is contrast? (No light = no image; no contrast = no resolution)
  2. Fluorescence and Stokes Shift
  3. Pixel Spacing (size?) Physical CCD dexels vs Picture Elements
  4. Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
  5. What is confocal
  6. Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
  7. Point Spread Function / Airy Disk
  8. lens cleaning
  9. safety ; mercury lamps, lasers (jewelry, interlocks)
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