Light Microscopy for You

Abstract here

its not a course OK?! What you need to know about light microscopy and the LMF/IPF but never knew to ask.

1 slide per point

1. What is a Microscope - Provdides Magnification and more importantly Resolution.
   1. Basic Ray Optics of a Lens.
   2. refraction
   3. Conjugate planes
      1. Single Lens has 2 focal planes FFP and BFP
      2. little microscope
      3. What is confocal
2. Resolution
   1. optical resolution - Point Spread Function / Airy Disk
   2. Pixel Spacing (size?) Physical CCD dexels vs Picture Elements vs point scanning
   3. Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
3. What is contrast? (No light = no image; no contrast = no resolution)
   1. Fluorescence and Stokes Shift
      1. Spectrum databases / viewers for planning expt.
4. Detectors: CCD vs. PMT Widefield vs point detection
   1. Digitization
   2. Detector Saturation
5. Objective language / reading
   1. Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
   2. Lens Immersion media
   3. old oil, wrong oil, wrong collar setting
   4. Lens cleaning
6. safety ; mercury lamps, lasers (jewelry, interlocks)
7. Project Planning / Workflow

1. Admin intro
   1. New User 30 min new user checklist