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== Confocal Infos and Introductions ==
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== Microscopy - Infos and Introductions ==
  
 
[[image:Primer-tirf.jpg|thumb|Molecular Expressions Microscopy Primer: TIRF introduction [http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfintro.html link]]]
 
[[image:Primer-tirf.jpg|thumb|Molecular Expressions Microscopy Primer: TIRF introduction [http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfintro.html link]]]
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* [http://support.svi.nl/wiki/NyquistCalculator Nyquist Calculator]<br>calculate the - according to Harry Nyquist - perfect sampling for your setup
 
* [http://support.svi.nl/wiki/NyquistCalculator Nyquist Calculator]<br>calculate the - according to Harry Nyquist - perfect sampling for your setup
<br>
 
  
== Spectral Data ==
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== Spectral & Optical Data ==
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=== Fluorochrome Spectra ===
  
 
[[image:Invitrogen-spectra.jpg|thumb|Invitrogen Spectra Viewer [http://probes.invitrogen.com/servlets/spectraviewer link]]]
 
[[image:Invitrogen-spectra.jpg|thumb|Invitrogen Spectra Viewer [http://probes.invitrogen.com/servlets/spectraviewer link]]]
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* [http://www.online-spectra.com/applet.html Online Spectra Viewer]<br>a lot of dyes, filters and lasers available, rather simple layout
 
* [http://www.online-spectra.com/applet.html Online Spectra Viewer]<br>a lot of dyes, filters and lasers available, rather simple layout
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=== Filter Specifications ===
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* [http://www.ahf.de/asp/group.asp?topnav=Filtersysteme AHF Analysentechnik Filtersystems]
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=== Objective Data ===
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* [http://www.zeiss.com/C12567BE0045ACF1/Contents-Frame/35ED128655E644D6C1256AD900473B53 Zeiss objective database]
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* [http://www.leica-microsystems.com/objectives.nsf/searchobjective?openform Leica objective database]
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* [http://microscope.olympus.com/uis2/ Olympus objective database]
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* [http://www.nikon.com/products/instruments/lineup/biological-microscopes/accessory/objective_lense/index.htm Nikon objective list]
  
 
== Free Software ==
 
== Free Software ==
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[[image:Micromanager-main panel.gif|thumb|µManager: main panel [http://micro-manager.org/ link]]]
 
[[image:Micromanager-main panel.gif|thumb|µManager: main panel [http://micro-manager.org/ link]]]
 
*[http://micro-manager.org/ µManager]<br>open source software package for imaging and control of automated microscopes, refer to the [http://valelab.ucsf.edu/~nico/MMwiki/index.php/Main_Page µManager Wiki] for configurations etc.<br>(Windows, Mac OS, Linux)
 
*[http://micro-manager.org/ µManager]<br>open source software package for imaging and control of automated microscopes, refer to the [http://valelab.ucsf.edu/~nico/MMwiki/index.php/Main_Page µManager Wiki] for configurations etc.<br>(Windows, Mac OS, Linux)
 
  
 
== Mailing Listserver ==
 
== Mailing Listserver ==
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* [http://rsb.info.nih.gov/ij/list.html ImageJ Mailing List]<br>lots of discussions, good if you are interested in using ImageJ more advanced
 
* [http://rsb.info.nih.gov/ij/list.html ImageJ Mailing List]<br>lots of discussions, good if you are interested in using ImageJ more advanced
 
  
 
== Companies ==
 
== Companies ==

Revision as of 09:14, 23 October 2008

Contents

Microscopy - Infos and Introductions

Molecular Expressions Microscopy Primer: TIRF introduction link
  • Nyquist Calculator
    calculate the - according to Harry Nyquist - perfect sampling for your setup

Spectral & Optical Data

Fluorochrome Spectra

Invitrogen Spectra Viewer link

Filter Specifications

Objective Data

Free Software

Image Processing and Analysis

ImageJ: colocalization analysis with automated threshold
  • BioImageXD
    open-source image analysis software
    (Beta, cross platform)
  • Zeiss ZEN LE
    new free Zeiss software for viewing and processing *.lsm images
    (Windows XP 32bit only)
  • Zeiss LSM Image Browser
    traditional free Zeiss software, can open database (*.mdb) and images (*.lsm) including all comments and acquisition information
    (Windows only)
  • Leica LAS AF Lite
    free Leica software, can open *.lif and *.lei files, acquisition information are accessible
    (Windows XP 32bit only)
  • Olympus FV Viewer
    free Olympus software, can open *.oib and *.oif files from Fluoview Confocals
    (Windows XP 32bit only)
  • Zeiss Axiovision LE
    free version of the Axiovision software, able to open *.lsm files as well, nice batch export
    (Windows only)
  • IrfanView
    image viewer for common file formats like *.tif and *.jpg, provides simple processing and batch conversion, slide show etc.
    (Windows only)

Image Acquisition

µManager: main panel link
  • µManager
    open source software package for imaging and control of automated microscopes, refer to the µManager Wiki for configurations etc.
    (Windows, Mac OS, Linux)

Mailing Listserver

  • ImageJ Mailing List
    lots of discussions, good if you are interested in using ImageJ more advanced

Companies

TILL Photonics: iMIC link
  • okolab
    stage incubators, cage incubators
  • Ibidi
    single- and multi-well dishes with cover-slip bottom, stage incubator