Links
From BioDIP
(Difference between revisions)
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* [http://www.online-spectra.com/applet.html Online Spectra Viewer]<br>a lot of dyes, filters and lasers available, rather simple layout | * [http://www.online-spectra.com/applet.html Online Spectra Viewer]<br>a lot of dyes, filters and lasers available, rather simple layout | ||
+ | |||
+ | <br> | ||
+ | == Free Image Processing Software == | ||
+ | |||
+ | * ImageJ<br>advanced open-source tool for image processing and analysis, lots of additional plugins available (cross platform) | ||
+ | McMaster Biophotonics Facility - Downloads | ||
+ | useful MBF ImageJ plug-in Bundle (continuation of WCIF bundle); also available: ImageJ Manual | ||
+ | LOCI Bio-Formats Library | ||
+ | plug-in for ImageJ, imports popular microscopy file formats, including those from confocal systems | ||
+ | o LSM Toolbox | ||
+ | plug-in for ImageJ especially for *.lsm files | ||
+ | |||
+ | * Improvision Volocity LE | ||
+ | easy-to-use image viewer for various microscopy file formats, including confocal ones | ||
+ | (Windows, Mac OS-X) | ||
+ | |||
+ | * BioImageXD | ||
+ | open-source image analysis software | ||
+ | (Beta, cross platform) | ||
+ | |||
+ | * Zeiss ZEN LE | ||
+ | new free Zeiss software for viewing and processing *.lsm images | ||
+ | (Windows XP 32bit only) | ||
+ | |||
+ | * Zeiss LSM Image Browser | ||
+ | traditional free Zeiss software, can open database (*.mdb) and images (*.lsm) including all comments and acquisition information | ||
+ | (Windows only) | ||
+ | |||
+ | * Leica LAS AF Lite | ||
+ | free Leica software, can open *.lif and *.lei files, acquisition information are accessible | ||
+ | (Windows XP 32bit only) | ||
+ | |||
+ | * Olympus FV Viewer | ||
+ | free Olympus software, can open *.oib and *.oif files from Fluoview Confocals | ||
+ | (Windows XP 32bit only) | ||
+ | |||
+ | * Zeiss Axiovision LE | ||
+ | free version of the Axiovision software, able to open *.lsm files as well, nice batch export | ||
+ | (Windows only) | ||
+ | |||
+ | * IrfanView | ||
+ | image viewer for common file formats like *.tif and *.jpg, provides simple processing and batch conversion, slide show etc. | ||
+ | (Windows only) | ||
+ | |||
+ | Mailing Listserver | ||
+ | |||
+ | * CONFOCAL@LISTSERV.BUFFALO.EDU | ||
+ | Confocal Microscopy List, many subscribers and discussions, archive function | ||
+ | |||
+ | * ImageJ Mailing List | ||
+ | lots of discussions – good if you are interested in using ImageJ more advanced |
Revision as of 16:36, 2 June 2008
Confocal Infos and Introductions
- Molecular Expression Primer
very comprehensive - provides information about various microscopy techniques, stainings and much more
- Jim Pawley Course Material
pre-course reading chapters foo Jim Pawley's "3D Microscopy of Living Cells" course
- Nyquist Calculator
calculate the - according to Harry Nyquist - perfect sampling for your setup
Spectral Data
- Fluorescent Spectra Database by Carl Boswell, George McNamara
comprehensive database with sorted spectras and lasers, lamps and filter sets
- BIO-RAD Microsciences fluorochrome database
fluorochrome spectra database, search function, Java based
- Invitrogen Fluorescence Spectra Viewer
nice layout, no search function, Java based
- BD Biosciences Fluorescence Spectrum Viewer
slightly different fluorochrome setup, i.e. some FACS dyes
- Online Spectra Viewer
a lot of dyes, filters and lasers available, rather simple layout
Free Image Processing Software
- ImageJ
advanced open-source tool for image processing and analysis, lots of additional plugins available (cross platform)
McMaster Biophotonics Facility - Downloads useful MBF ImageJ plug-in Bundle (continuation of WCIF bundle); also available: ImageJ Manual LOCI Bio-Formats Library
plug-in for ImageJ, imports popular microscopy file formats, including those from confocal systems o LSM Toolbox plug-in for ImageJ especially for *.lsm files
* Improvision Volocity LE easy-to-use image viewer for various microscopy file formats, including confocal ones (Windows, Mac OS-X)
* BioImageXD open-source image analysis software (Beta, cross platform)
* Zeiss ZEN LE new free Zeiss software for viewing and processing *.lsm images (Windows XP 32bit only)
* Zeiss LSM Image Browser traditional free Zeiss software, can open database (*.mdb) and images (*.lsm) including all comments and acquisition information (Windows only)
* Leica LAS AF Lite free Leica software, can open *.lif and *.lei files, acquisition information are accessible (Windows XP 32bit only)
* Olympus FV Viewer free Olympus software, can open *.oib and *.oif files from Fluoview Confocals (Windows XP 32bit only)
* Zeiss Axiovision LE free version of the Axiovision software, able to open *.lsm files as well, nice batch export (Windows only)
* IrfanView image viewer for common file formats like *.tif and *.jpg, provides simple processing and batch conversion, slide show etc. (Windows only)
Mailing Listserver
* CONFOCAL@LISTSERV.BUFFALO.EDU Confocal Microscopy List, many subscribers and discussions, archive function
* ImageJ Mailing List lots of discussions – good if you are interested in using ImageJ more advanced